circPRKAA2通过调控miR-22-3p/SLC27A1轴对奶牛乳脂合成的影响

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中图分类号：S823.8 文献标志码：A 文章编号：0366-6964（2026）02-0740-13

Effect of circPRKAA2 on Milk Fat Synthesis in Dairy Cowsvia the miR-22-3p/ SLC27A1Axis

WANG Chuanchuan 1，2 ，CHEN Yafei 1，2 ，TIAN Jia³，FENG Xiaofang4，TONG Lijia 1，2 ，WANG Hua 1，2 XUE Lin 1，2 ，GUYaling .1，2 ， ZHANG Juan 1，2 *

（1. College of Animal Science and Technology，Ningxia University， Yinchuan 75O021， China; 2. Ningxia Hui Autonomous Region Key Laboratory ofMolecular Cell Breeding， Yinchuan 75O021，China; 3. Ningxia Hui Autonomous Region Animal Husbandry Workstation， Yinchuan 75OO21， China; 4. College ofLife Sciences and Engineering，Northwest Minzu University，Lanzhou 73Oo3O，China）

Abstract： The aim of this study was to explore the impact of molecular networks constructed by DEcircRNAs related to milk fat synthesis on milk fat synthesis in dairy cows through in-depth analysis. In this study，we used the circRNA library and transcriptome sequencing data of Holstein dairy cows with high and low milk fat percentage constructed previously to screen and identify DE-circ RNAs （circPRKAA2） related to milk fat synthesis，and further predicted their ceRNA regulatory network using Targetscan and miRanda software.Real-time fluorescence quantitative PCR，RNA pull down assay and dual luciferase reporter assay were used to verify the targeting relationship. Oil red O and BODIPY staining were used to investigate the efect of target miRNA on lipid droplet secretion in BMECs. The results indicated that the regulatory relationship of the ceRNA （circPRKAA2/miR-22-3p/ SLC27A1） network was successfully predicted and verified，and 9 miRNAs and 1O candidate mRNAs were predicted，and it was clarified that circPRKAA2 and miR-22-3p were mainly distributed in the cytoplasm of BMECs. circPRKAA2 negatively regulated miR-22-3p during the process of milk fat synthesis in BMECs. miR-22-3p inhibited the secretion of lipid droplets，the production of triglycerides and cholesterol in BMECs，and negatively regulated SLC27A1. In conclusion，this study analyzed and verified the targeting relationship of circPRKAA2/miR-22-3p/ SLC27Al and its regulatory effect on milk fat synthesis，with the hope of providing new insights for improving milk quality.

Keywords：Holstein cow;SLC27A1;miR-22-3p;circPRKAA2;milk fat *Corresponding author： ZHANG Juan，E-mail： zhangjuannxy@nxu. edu.cn

乳脂是一种高质量的天然脂肪，不仅是影响牛奶品质的关键因素，也是反刍动物育种的主要目标性状[1]。（剩余20108字）