旋毛虫核激素受体NHR-23克隆表达和特性分析

打开文本图片集

中图分类号：S852.731；R383.15 文献标志码：A 文章编号：0366-6964（2026）02-1034-12

Cloning，Expressionand CharacterizationofTrichinella spiralis Nuclear Hormone Receptor NHR-23

Abstract： This study aims to evaluate the biological characteristics of Trichinella spiralis nuclear hormone receptor-23（NHR-23）and its potential for vaccine development. The recombinant TsNHR-23 protein （rTsNHR-23） was obtained via cloning and expression，followed by purification. Its immunoreactivity was assessed via Western blot，while transcriptional levels of the TsNHR-23 gene across different developmental stages of T ：spiralis were analyzed using qPCR. In vitro experiments were conducted to analyze the inhibitory effect of anti-rTsNHR-23 polyclonal antibody on the molting of muscle larvae and to explore the efect of this protein on the function of mouse macrophages. The immune-protective efcacy of PLGA nanoparticle-encapsulated rTsNHR-23 was tested by immunizing ICR mice and analyzing parasite burden. The TsNHR-23 gene was transcribed across all developmental stages of T ：spiralis，with the highest expression observed inmuscle larvae （ML） stage. Anti-rTsNHR-23 antibodies significantly reduced larval molting rates and inhibited molting progression. The rTsNHR-23 protein significantly enhanced the transcription levels of IL- 1β ， IL-6，and IL-1O in mouse macrophages. Immune protection experiments demonstrated that mice immunized with the rTs-NHR-23 protein exhibited a highly significant increase in serum IgG antibody levels compared to the control group.After the first immunization，IGF-1 levels markedly decreased，while IL-6 and TGF- β levels increased． Following the second immunization，IGF-1 partially rebounded，whereas TGF- β showed significant downregulation. Post-challenge infection， compared to the control group，the immunized mice displayed significant reductions in the number of T. spiralis adult worms，the average number of newborn larvae produced per female worm，and the muscle larval burden. rTsNHR-23 exhibits potent inhibitory effects on ML molting，modulates macrophage functions，and significantly reduces parasitic load in immunized hosts，demonstrating promising potential as a candidate vaccine against T ，spiralis.

Keywords： T. spiralis;nuclear hormone receptor;macrophage;immune protection *Corresponding author： YAN Ruofeng，E-mail： yanruofeng@njau. edu. cn

旋毛虫病是由旋毛形线虫引起的食源性寄生虫病1，对人类健康构成严重威胁。（剩余19342字）