牛胎盘DLK1-DI03区域的印记及甲基化状态研究
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中图分类号：S823.2 文献标志码：A 文章编号：0366-6964（2026）02-0789-12
Analysis of ImprintingandDNA Methylation Status ofDLK1-DIO3Domain in Bovine Placenta
ZHENG Yunchang1，HOU Ruilin1，LIANG Xiaohe¹，WEI Zilu1，YANG Lidan 1 ，HUO Haonan 1 ， SU Shuoqing²，ZHANG Cui¹，LI Shujing²*，LI Shijiel* （1. College of Life Science， Hebei Agricultural University，Baoding O71Ool， China; 2.Hebei Tianhe Beef Cattle Seed Industry Co.，Ltd.，Shijiazhuang O5O2OO，China）
Abstract： The objective of this study was to investigate the imprinting status and to search for potential DNA differentially methylated regions （DMRs） of the DLK1-DIO3 domain in bovine placentas. In this study，whole genome sequencing （WGS） was performed on 3 bovine placentas and their parents to mining the informative single nucleotide polymorphisms （SNPs）. The imprinting status of genes in the DLK1-DIO3 domain were determined by the combined analysis of WGS and transcriptome sequencing （RNA-seq） results. The DMRs were identified by combination of WGS and whole genome bisulfite sequencing（WGBS） data. WGS and RNA-seq results showed that MEG3，MEG8 and MEG9 genes were maternally expressed imprinted genes and DLK1，RTL1 and DIO3 genes were paternally expressed imprinted genes. The WGBS data analysis showed that 4 DMRs，Dlk1-DMR，IG-DMR，Gtl2-DMR and Meg8-DMR were conserved in human and mouse， and 5 new DMRs were identified，DLK1-DMRO and DLK1-DMRl located upstream of the DLK1 gene，MEG9-DMR in MEG9 promoter region， and DIO3-DMRl and DIO3-DMR2 located upstream of DIO3 genes，respectively. The results indicate that the imprinted status and differentially methylated regions of bovine DLK1-DIO3 imprinted region are highly conserved to human and mouse.In addition，5 potential DMRs were identified in bovine DLK1-DIO3 region，and their regulating mechanism on the DLK1-DIO3 imprinted region needs to be further investigated.
Keywords： DLKl-DIO3 imprinted domain; transcriptome;methylome; bovine; placenta ∗ Corresponding authors：LI Shijie，E-mail： lishijie2O005@163.com；LI Shujing，E-mail： embryochina@163.com
基因组印记（genomicimprinting）是发生在哺乳动物中的一种表观遗传学机制，是指在子代中仅表达来源于亲本一方的等位基因，而另一方不表达或很少表达的现象[1]。（剩余16851字）
