间歇性限饲通过自噬改善小鼠卵巢功能的研究

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中图分类号：S814.1 文献标志码：A 文章编号：0366-6964（2026）02-0801-09

Intermittent Feed Restriction Improves Mouse Ovarian Function through Autophagy

CHEN Xinyan1，LU Jiajia¹，XU Chenchen1，TAO Yue1，XUE Tongmin 2 ，LIFeng²， ZHANG Xinjun1*， JU Huimingl* （1. Jiangsu Co-innouation Center for Prevention and Control ofImportant Animal Infectious Diseases and Zoonoses， College of Veterinary Medicine， Yangzhou University， Yangzhou 225OO9，China; 2.Reproductive Medicine CenterofNorthern Jiangsu People's Hospital Affiliated，Yangzhou 225O09，China）

Abstract：This study adopted an IF feeding method to investigate the effects and mechanisms of this dietary approach on ovarian function in mice，aiming to provide a theoretical basis for livestock reproduction management. In this study，8-week-old female C57BL/6J mice with similar body weights were randomly divided into two groups： one group served as the normal diet control group （ND），while the other group was subjected to a cyclic pattern of“1 day of restricted feeding （ 70% feed intake）-1 day of free feeding”to establish an intermittent mild fasting （IMF） mouse model. The growth performance of the mice was measured，and ovarian function as well as the expression of autophagy-related proteins were detected through Western blot （WB）and immunohistochemistry experiments. The results showed that IMF could significantly reduce the feed-to-gain ratio （82.85± 2.68&.93.02±7.40） ）and abdominal fat index （ 1.49±0.118.1.72±0.19） . At the same time，the autophagy markers LC3 II /LC3 I （1.06±0.12& 0.89±0.02） and reproductive function proteins BMP-15（1.05±0.08& 0.75±0.10）and GDF-9 （0.53±0.02 & 0.44±0.02 ）were upregulated. The mechanism study showed that IMF activated autophagy by inhibiting mTOR pathway p-mTOR/mTOR0.82±0.10&0.97±0.05） ，and then improved ovarian function. This study is the first to elucidate the mechanism by which IMF enhances ovarian function through autophagy activation， offering a potential non-pharmaceutical strategy for developing reproductive regulation techniques in livestock.

Keywords： intermittent feed;autophagy; ovarian function; livestock reproduction ∗ Corresponding authors： JU Huiming，E-mail： hmju@yzu.edu. cn； ZHANG Xinjun，E-mail ： zhangxj@yzu. edu. cn

随着畜牧产业向高效化、生态化方向发展，如何通过营养策略改善家畜繁殖性能已成为研究热点[1]。（剩余14104字）