鼠乳杆菌ZNL-13对环磷酰胺诱导犬免疫抑制的效果观察

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中图分类号：S852.4 文献标志码：A 文章编号：0366-6964（2026）02-1110-14

Evaluation of the Effects of Lactobacillus murinus ZNL-13 on CyclophosphamideInduced Immunosuppression in Dogs

DONG Yihan 1，2 ，YAO Renxin1，JIA Haitao1，WANG Haiyang1，ZHANGLuyao 1 ，XIEYushan1， WANG Hanyu1， ZHANG Jiantao1，2* （1.College of Veterinary Medicine，Northeast Agricultural University，Harbin 15OO3O，China; 2. Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and ComparativeMedicine，Harbin150O3O，China）

Abstract： Cyclophosphamide （CTX） is a widely used chemotherapeutic agent for treating canine tumors；however，its potent immunosuppressve effects can impair immune function and disrupt gut microbiota homeostasis， increasing the risk of secondary complications. Lactobacillus murinus （L. murinus）has been reported to modulate immune cell activity and protect the gastrointestinal tract， highlighting its potential as a functional food additive. In this study，a probiotic strain with optimal characteristics，L. murinus ZNL-13，was isolated from the fresh feces of healthy dogs. The aim was to evaluate the efects of this canine-derived strain on CTX-induced immunosuppression and gut microbiota dysbiosis in dogs. Serum immune parameters and fecal 16S rRNA sequencing were used for analysis. Results showed that prophylactic administration of L . murinus ZNL-13 significantly improved CTX-induced reductions in white blood cells and lymphocytes （ ⋅P<0.05 ），alleviated liver and kidney injury，reduced oxidative stress （ P<0.05 ），and enhanced intestinal barrier function by lowering serum levels of DAO and D-lactic acid （ P<0.01 ）．In addition，L. murinus ZNL-13 mitigated the CTX-induced decline in serum IgG and key immune cytokines （ ⋅<0.05 ），increased the abundance of beneficial gut bacteria，and restored microbial homeostasis. Meanwhile，the levels of beneficial bacteria such as Blautia_A，Holdemanella，and Peptacetobacter were increased， contributing to the restoration of gut microbiota homeostasis. In conclusion，L. murinus ZNL-13 effectively counteractsCTX-induced immunosuppresson， oxidative stress， and gut barrier dysfunction，ofering a promising strategy for the development of novel functional food additives to support immune health in dogs.

Keywords：Lactobacillus murinus; immunosuppression;gut microbiota * Corresponding author： ZHANG Jiantao，E-mail： zhangjiantao@neau.edu.cn

环磷酰胺（cyclophosphamide，CTX）是目前临床上广泛使用的抗癌药物，常用于白血病、癌症等疾病的治疗[1]。（剩余23819字）