双氢青蒿素通过Ras通路抑制胶质母细胞瘤的恶性进展

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【中图分类号】 R739.41 【文献标志码】A 【文章编号】 1672-7770（2025）06-0643-06

Abstract： Objective Toinvestigate the mechanism of dihydroartemisinin（DHA）in glioblastoma（GBM），using a combination of network pharmacology and cellular experiments.MethodsPotential targets of DHA and GBM were obtained from databases，and a DHA-GBM interaction network was constructed using String. overlapping target genes were subjected to Gene Ontology（GO）and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway enrichment analyses. Subsequently，in vitro experiments，including CCK-8 assay，flow cytometry apoptosis detection，and Western blotting，were performed to assess the inhibitory effects of DHA on GBM and its underlying mechanisms.ResultsIntersection analysis identified shared targets between DHA and GBM. GO and KEGG enrichment analyses revealed key signaling pathways involved. In vitro experiments demonstrated that DHA significantly inhibited the proliferation，migration，and invasion of U87 and U251 cells while inducing apoptosis.Western blotting experiments suggested that DHA suppressesthemalignantprogressionofGBMthroughtheRassignalingpathway.ConclusionsThis study explores the potential targets and related pathways of DHA therapy for GBM based on network pharmacology， providing a theoretical basis for promoting DHA therapy for GBM in the future.

Key words：dihydroartemisinin；glioblastoma；Ras pathway；network pharmacology

神经胶质瘤作为起源于神经上皮组织的肿瘤，约占所有脑肿瘤的 40%～50% ，是颅内最常见的原发性恶性肿瘤之—[1-2]。（剩余10640字）