红景天苷通过VIP-cAMP通路对大鼠腮腺放射性损伤的改善作用及机制

打开文本图片集

中图分类号R965 文献标志码A 文章编号 1001-0408（2025）22-2796-06

DOI 10.6039/j.issn.1001-0408.2025.22.08

ABSTRACTOBJECTIVETo explore theimprovement efectand mechanismof salidrosideonradiation-induced parotid gland injury inrats.METHoDsRats wererandomly asigned into normal group，radiation group，salidroside low-dose（salidroside-L， 50mg/kg ）group，salidroside high-dose（salidroside-H， 100mg/kg ）group，and salidroside-H+inhibitor（ 100mg/kg salidroside +0.1 μmol/kgH-θ9 ）group，with10 rats in each group.Except forthe normal group，rats in the other groups were subjected to radiation exposure toestablishamodelofradiation-inducedparotidglandijury.Rats ineachgroupwereintraperitoneallinjectedwiththe corresponding drugornormal saline，onceaday，for40consecutivedays.After thelastadministration，thelevelsofeactive oxygen species（ROS），cyclic adenosinemonophosphate（cAMP），superoxide dismutase（SOD），and amylase in serum were detected；thepathological changes of parotidglandtissewereobserved;theapoptosisrateof parotidglandtisuecells，the expressionlevelsofB-cellymphoma-2（Bcl-2）anditsassociatedXprotein（Bax），mRNAexpressionlevelsof interleukin-6（- 6）and tumor necrosis factor ⋅α （TNF- α⋅∝ ），the protein expression levels of type Il collagen（（Col II），vasoactive intestinal peptide （VIP），andthephosphorylationlevelof protein kinaseA（PKA）inparotidglandtisse were determined.RESULTSCompared with normal group，thelevelsofROS，amylase，apoptosisrate，Bax expresion level，mRNA expressonlevelsofIL-6andTNFα，and protein expression level of Col II in the radiation groupweresignificantly increased，while thelevelsofcAMP， SOD，Bcl-2 expression level，VIP protein expression level and PKAphosphorylation level were significantly decreased （ P< 0.05）.Compared with radiation group，the above indicators in the salidroside-L group and salidroside-H group were significantly improved （P<0.05） ），and the improvement in the salidroside-H group was more significant （ （P<0.05 ）；inhibitor H-89 significantly reversed the changes in the above indicators of the salidroside-H group（ ⋅<0.05 ）.CONCLUSIONS Salidrosidecan effectivelyalleviateradiation-induced parotid gland injuryin rats，and its mechanism may be related to the activation of the VIP-cAMP pathway.

KEYWORDSsalidroside；radiation injury；parotid gland;VIP-cAMP pathway

放疗是治疗头颈部肿瘤最常用的手段之一，在鼻咽癌、口腔癌、甲状腺癌的治疗中尤为重要。（剩余10657字）