当归有机酸的低共熔溶剂提取工艺优化及机制研究

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中图分类号R284.2 文献标志码A 文章编号 1001-0408（2025）22-2783-07

DOI 10.6039/j.issn.1001-0408.2025.22.06

ABSTRACTOBJECTIVE Tooptimize theextraction processof organicacids from Angelica sinensisusing deep eutectic solvents（DESs），andconductcharacterization，antioxidantactivity evaluation，and extraction mechanismanalysis.METHODs Theconductor-likescrening modelforrealisticsolvation with segmentactivitycoeficients（COSMO-SAC）wasemployedto screenthetypesofDESs.Withtotalorganicacidcontentastheresponsevalue，single-factorexperimentsandBox-Behnken response-surface methodologywereused tooptimize theextractionconditions.UsingA.sinensis decoctionpieces and/orA.sinensis methanolextractasreferences，scaningelectronmicroscopeandFourier transforminfrared spectrometer（FTR）wereappliedto characterize the products.Additionally，the 1，1-diphenyl-2-picrylhydrazyl（DPPH）and 2， 2′ -azino- .bis （3-ethylbenzothiazoline-6- sulfonicacid）（ABTS）freeradicalscavengingcapacitiesweredetermined.Densityfunctionaltheory（DFT）wasusedtoanalyze theextractionmechanismofferulicacidandchlorogenicacidbytheDESs.RESULTs&CONCLUSIONSTheoptimalDESs was choline chloride-propanediol.The optimal extraction conditions for organic acids from A .sinensiswere as follows：choline chloridepropanediol molar ratio of 1：1，DESs water content of 70% ，solid-liquid ratio of 1：10 ，heating temperature of 57∘C ，and heating and stirring time of 8 min. In three validation experiments，the total organic acid content was 2.92mg/g ，yielding a relative error of 0.34% compared to the predicted value （ 2.91mg/g⟩ .Compared with A ，sinensis decoction pieces and methanol extracts，the agglomerated structureoftheDESs extract powderalmost disappeared，showing the presenceoflamelarstructures similartothose oftheintestinalwall.Comparedwiththemethanolextract，theDSextractexhibitedhigherFTRcharacteristicpakitensityand peak areaintegration，aswellasstronger scavengingcapacities against DPPH and ABTSfreeradicals.Theextractionof organic acidsfrom A .sinensis byDESsisthe resultofthecombined effectsofpolaritymatching，hydrogen bonding，and structural adaptation.

KEYWORDS Angelica sinensis；deep eutectic solvents; ferulic acid；chlorogenic acid；COSMO-SAC；Box-Behnken response-surface methodology；processoptimization

当归是伞形科植物当归Angelicasinensis（Oliv.）Diels的干燥根，始见于《神农本草经》，性温，味甘、辛，归心、脾、肝经，有“血中圣药”之称，可用于血虚、月经不调、便秘、腹痛、风湿痹痛等症。（剩余12146字）