基于血清药物化学与网络药理学的鸡骨草和毛鸡骨草抗肝炎作用机制研究

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中图分类号R284；R285 文献标志码A 文章编号 1001-0408（2025）22-2772-06

DOI10.6039/j.issn.1001-0408.2025.22.04

ABSTRACTOBJECTIVETocompare theanti-hepatitismechanismsofAbruspulchellussubsp.cantoniensis（Hance）Verdc （AC）and Abrus pulchelussubsp.molis（Hance）Verdc.（AM）.METHODs SDrats were randomly divided into blank group，ACtreated group，andAM-treatedgroup，witheachgroupconsistingof1Orats.Therats’orbitalvenous bloodwascolectedat5，15， 30 minutes，and 1，1.5，2，4，6，8，12 hours after gavage administration of 24g/kg of the corresponding drug （calculated by crudedrug）orwater，respectively.Utra-performanceliquidchromatography-quadrupoletime-of-flightmassspectrometry technologywasutilizedtoidentifytheprototypecomponentspresentintheserum.Thenetworkpharmacologymethodwasadopted topredicttheanti-hepatitisactivecomponents，keytargets，andsignalingpathwaysofACandAM.Aditionally，molecular docking technologywasuilizedtoverifythebindingactivityofthecoreactivecomponentswithkeytargets.RESULTSAtotalof 35prototypecomponents migrating tothe bloodofACandAM wereidentified in theserumofadministeredrats，among which 24 were commoncomponents.The activecomponents inAC，suchasacetylanguidine，physcion，soyasaponinA3andsoyasaponinI，aswell asthose inAM，includingvicenin3，acetylanguidine， soyasaponin I and schaftoside，all acted on key targets such assteroid receptor coactivator， phosphatidylinositol-4，5- bisphosphate 3-kinasecatalyticsubunit alpha， epidermal growth factor receptor（EGFR），and protein kinase B1 （Aktl）. These components modulated pathways in cancer， EGFRtyrosinekinaseinhibitorresistance，andthe phosphoinositide3-kinase（PI3K）-Aktpathway，thereby exerting anti-hepatitis effects. Furthermore， thebinding energiesbetween these activecomponents and their key targets were all less than .CONCLUSIONS There are differences in the active components of AC and AM against hepatitis， buttheirmechanismsofactionaresimilar.Bothmayexerttheiranti-hepatiseffectsthroughpathwaysincancer，EGFRtyrosine kinase inhibitor resistance，and the PI3K-Akt pathway.

KEYWORDsAbrus pulchelussubsp.cantoniensis （Hance）Verdc.；Abrus pulchelusubsp.molis （Hance）Verdc.；anti-hpatitis; serum pharmacochemistry；network pharmacology；molecular docking；mechanism of action

鸡骨草为豆科植物广州相思子Abruspulchellussubsp.cantoniensis（Hance）Verdc.的干燥全株，又名小叶鸡骨草、黄头草和黄仔强等，性凉，味甘、微苦，具有利湿退黄、清热解毒、疏肝止痛的功效，主要分布于我国广东、广西等地，系广西"桂十味"道地药材。（剩余10995字）