添加不同比例生物质炭对设施土壤细菌群落结构的影响

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中图分类号：S154.3 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2025.04.007

Abstract：To investigate the effects of different biochar addition ratios（ 0% ，1%， 2% ， 3% ， 5% ）on the bacterial community structure anddiversityinfciltycultivatedsoil，thisstudyconductedapotexperimentusingrecycledflowercontinuouscopingsoil.ByemployingIluminaMiseqhig-throughputsequencingtechnologyandAlphaandBetadiversityanalysis，theregulatoryefectsofbiochar additiononbacterialcommunitiesweresystematicallexplored.Theresultsdemonstrated thatbiocharaplicationsignificantlyenhanced soil bacterial Alpha diversity，with an average increase of 19.85% in Chaol index，a maximum increase of 10.3% in Shannon index and a slight increase in Simpson index ，particularly under the 5% addition ratio treatment（T4）.At the phylum level，the relativeabundancesofProteobacteria，cidobacteriotaandActinobacteriotaincreasedwhilethatofFirmicutesdecreased.Genus-level analysisrevealedsignificantenichmentofbeneficialbacteriasuchasBacilus.Principalcoodinateanalysis （CoA）indicatedsignificant structural differences among treatments（ R2=55.83% ）.In conclusion，the study elucidates that biochar optimized bacterial communityfunctionalitythroughmultiplemechanisms：providingbacterialhabitatsviaitsporousstructure，promotingheterotrophicbacte ria proliferation through slow-release carbon sources，and suppressing pathogens via toxin adsorption. The 5% addition ratio treatment achievesoptialimprovementinsoilbacterialimbalane.Tisresearchprovides teoeticalsupportforbacterialapplicatioam liorating continuous cropping facility cultivated soil.

Key Words： biochar; facility cultivated soil; bacterial community; high-throughput sequencing

土壤微生物群落是驱动土壤养分循环、有机质转化和生态系统稳定的核心生物因子，细菌和真菌作为核心功能类群，通过协同与竞争关系驱动着关键的生态过程。（剩余8951字）