大球盖菇液体菌种最佳配方筛选

打开文本图片集

中图分类号：S646 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2025.04.00

Abstract：InordertoimprovethefciencyofproducingliquidspawnofStrophariarugosoaulata，ascreningmethodwasusedto conductatriangularflaskcultureexperimentand9orthogonalexperimentsonthebasicliquidstrainformulasofStrophariarugosoanulatawithdiferentnitrogensourcecompositions.Theresultsshowedthatinthesinglefactorexperiment，thenitrogensourceswith highermycelialbiomasswerebranandsoybeanpowder.Thevarietywiththehighestmycelialbiomassinthetriangularflaskwasthe DS-2breedofStrophariarugosoannulata，ndthemediumwiththehighestaverage mycelialbiomasswasPDB（potatoglucose）medium.Byculturing theoptimal DS-2bredinatriangularflask，theoptimalrangeforaddingsoybeanpowder was determined tobe 5- （204号 15g⋅L-1 ，and the amount of bran added was determined to be 0-10g⋅L-1 respectively. On this basis，potato and glucose were used as carbonsources，ndafourfactorthree-levelorthogonal experimentaldesignwasconductedtoobtaintheoptimalformula，whichwas tested and proved to be 5g of bran， 15g of soybean flour，10 g of glucose，200 g of potato，1 g of potassium dihydrogen phosphate， 0.5 gof magnesium sulfate，and 1000mL of ultrapure water.The maximum dry mass of mycelium reached 1.32g⋅100mL-1 ，and the averagediameterwaslessthan2mm.Theexplorationof extracelularenzymeactivityshowedthat theoptimal innineformulahad the lowest laccase activity，which was 0.5360U⋅mL-1 ; On the contrary，the formula with the lowest dry mass of mycelial had the highest laccase activity，peaking at 7.195U⋅mL-1 ; There was a significant negative correlation between cellulase activity and biomass， R=- 0.692.Insummary，theoptimal formula for liquid spawnof Strophariarugosoannulatais acompositionof5gof bran，15 g soy powder， 10g glucose and 200g potato slices， KH2PO4 for1 g and MgSO4 O.5 g，in 1 liter ultrapure water. This formula has high dry biomass， lowcost，andsimpleconfiguration.Sincethefomulahasthebovecharacteristics，itcanbeappliedtoliquidspawnseedtankfor further exploration and research.

Key words： Strophria rugosoannulata; liquidspawn;optimal formula; extracellular enzyme activity

大球盖菇（Strophariarugosoannulata），别称酒红色球盖菇，皱环球盖菇，赤松茸等，在分类学上，属微生物界担子菌门（Basidomycota）层菌纲（Hymenomycetes）伞菌目（Agaricales）球盖菇科（Strophariaceae）球盖菇属（Stropharia）[]。（剩余10428字）