综合转录组和代谢组分析揭示嫁接提高烟草钾利用效率的分子机制

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中图分类号：S572 文献标识码：A DOI 编码：10.3969/j.issn.1006—6500.2025.04.001

Abstract：Toclarifythemolecular mechanismoficreased potassiumcontentinobacoleavesafter grafting，combinedapproachof transcriptomicsand metabolomics wasadopted.Thepotassum-efcientvariety Wufeng No.2andthemaincultivatedtobaccovariety Yunyan87wereselectedastebects tostudytheolgicalbasisoftedierencsinpotassumutilizaiofecaogint tobacco rootstocks.The results showed a considerable increment of 90.1% in the potassium content of the grafted tobacco leaves than Yunyan 87 （ P <0.05）.Overall，2044 diffrentially expressed genes were identified through transcriptome analysis，with themajority being enrichedin plant hormone signal transductionandthe MAPKpathway.Metabolomeanalysisrevealed175metabolites with significantdiferences，primarilyinvolving primarymetabolitessuchasaminoacidsandcarbohydrates.Amongthese，there wasan increaseinthemetaboliteslevelsrelatedtoglycolysis，aminoacidmetabolism，ndtheTCAcyclepathwayingraftedtobacoleaves. The keymetabolitesand genes intheabovepathways wereselectedfor ManteltestandPearsoncorelationanalysis，leading tothe identification of 2 genes and 3 metabolites，including IAA ，CIPI，D-fructose，Fumaric acid and Oxoglutaric acid，that were significantly associated with the increased potassium content in grafted tobacco（Mantel R>O.8 or Mantel R<-0.2， P <0.05）.In conclusion， theup-regulationofpotassmsigaltansductionrelatedgnes，longwithasubstantialincreaseinpimarymetaboliteslikanic acidsand sugars，are crucial determinants for the eficient absorption and utilizationof potassium in grafted tobacco.

Key words：grafting;metabolome;potassium; tobacco;transcriptome

钾（K）元素是显著影响烤烟叶片质量的必需营养元素。（剩余19642字）