百香果果生炭疽菌小GTP酶基因Rab7的鉴定与功能分析

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关键字：百香果；果生炭疽菌；小GTP酶基因Rab7；致病性中图分类号：S436.639 文献标识码：A 文章编号： 1000-4440（2025）06-1107-09

Abstract：Anthracnose of Passflora edulis caused by Coletotrichum fructicola isa significant disease afecting the yield andqualityof pasionfruit.Elucidating thepathogenicmolecularmechansmsofColetotrichumfructicolaisof greatsignificance for disease control. In this study，we identified the small GTPase Rab7 from c . fructicola and compared its amino acid sequences withthoseof Rab7 proteins from various fungi.Phylogeneticanalysis revealed that theRab7proteinof c fructicola hadthemost distantrelationshipwiththeRab7proteinofScharomycescerevsiae，whileitwasloselyelatedtotheRab7proteinsofColeto trichumgloeosporioidesand Colletotrichumgraminicola.Using homologous recombination technology，we constructed the Rab7 gene knockout strain ΔRab7 and the complemented strain △Rab7/Rab7.Phenotypic analysis showed that comparedwith thewild type and Δ Rab7/Rab7，the Δ Rab7 mutantexhibited significantlyreducedcolonydiameter on PDAY and MM media，significantly increased melanin accumula

tion，and significantly decreased conidial production.However， Δ Rab7and the wild type N425did not show significant differences in sensitivity to H2O2 stress.Confocal laser scanning microscopy observations indicated that the Rab7-GFP fusion protein was localized to the vacuolar membrane，and the vacuoles in Δ Rab7appeared fragmented，suggesting that Rab7 gene maintained normal vacuolar morphology in c fructicola by regulating vacuolar fusion.This study provides a theoretical basis for elucidating the molecular mechanisms of pathogenicity in C* fructicola.

Key Words： Passiflora edulis；Coletotrichum fructicola；small GTPase gene Rab7；pathogenicity

百香果（Passifloraedulis），又称西番莲、鸡蛋果，是西番莲科西番莲属的多年生常绿攀缘草质藤本植物，主要分布于热带和亚热带地区，在中国广西、、广东和海南等地广泛种植，其果汁色泽鲜艳，酸甜可口，富含营养，具有较高的食用价值、观赏价值、药用价值，深受消费者喜爱[1-3]。（剩余11067字）