低温胁迫下玉米根系转录组和代谢组分析

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中图分类号：S513.01 文献标识码：A 文章编号：1000-4440（2025）06-1063-09

Abstract：Cold stress is one of the primary abiotic stresses limiting the growth of maize seedlings.Inorder to study theeffectoflowtemperaturestressonmaizeroots，thisstudyusedmaizeinbredlineB73astheexperimentalmaterial，and dynamically monitored the growth of the primary root at different time points under control（28 C day/22 C night）and low-temperature treatment （ 15‰ day/ 10qC night）.Additionally，we determined the transcriptome and metabolome of the rottissues from both thecontrol groupand the1-daylow-temperature treatment group.Phenotypic characterizationresults indicatedthatthe growthof the primaryroot was significantly inhibitedunderlow-temperature stress.Metabolomic analysis identified 57diferentiallyaccumulated metabolites，predominantlyenriched in starchand sucrose metabolism pathways.A total of 2 769 differentially expressed geneswere identified bytranscriptomeanalysis，which weremainlyinvolved in starch and sucrose metabolism and phenylalanine metabolism.Further integrationof transcriptomic andmetabolomic analyses revealed that starch and sucrose metabolismwere keypathwaysmediatingroot adaptationtolow-temperature stress.Under low-temperature stress，the content of soluble

sugarssuchasfructoseandglucosesignificantlyincreasedAtotalof26diferentiallyexpressed genes wereidentifiedinthe starchand sucrose metabolicpathways，with12 genesshowing significant upregulationand14 genesshowing significant downregulation.Thisstudyprovidesatheoretical foundationandcandidategenes forfurther investigation intothemolecular mechanisms of maize root adaptation to low-temperature stress and for genetic improvement.

Key words： maize；low temperature stress；transcriptomics；metabolomics

植物的生长和发育依赖于农田环境，在植物生长过程中常受到干旱、高温、低温、洪涝等非生物胁迫的影响[1]。（剩余15595字）