甘蓝型油菜OSCA家族基因鉴定及其在非生物胁迫下的表达分析

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中图分类号：S656.4 文献标识码：A 文章编号：1000-4440（2025）06-1050-13

Abstract：Hyperosmolarity-gate calcium-permeable channels（OSCA）playa significant role in the response of plants to abiotic stress.To elucidate the response mechanism of BnaOSCA family members under abiotic stress，this study identified 37 OSCA genes from the whole genome of Brassica napus and analyzed the gene structure，protein properties，and expresion paterns.The results showed that these 37 OSCA genes were unevenly distributed on 13 chromosomes and could bedivided intothreesubfamilies.Membersof thesamesubfamilyhadconservedgenestructuresandconserved motifs，and thepromoter regions of BnaOSCA family genes contained avariety of cis -actingelementsrelated to abioticstressand hormoneresponses.The collinearity analysisresults indicatedthat gene loss ocurredduring theevolutionof the BnaOSCA family genes，and gene family expansion also took place.Undersaltanddrought stress，therelative expresion levelsof BnaOSCA family genes varied，indicating that BnaOSCA family genes played diferent regulatory roles inthe response to abiotic stress.This study provides a basis for further perfecting the regulatory network of BnaOSCA family genes.

Key words： Brassica napus；OSCA family genes；drought stress；salt stress；expression pattern

钙通透性阳离子通道蛋白OSCA（Hyperosmolar-ity-gatecalcium-permeablechannels）是一类非选择性通道，作为植物中首个被鉴定的渗透压感受型钙离子通道，在感知胞外渗透压变化和调控植物生长发育中发挥重要作用[1-3]

在模式植物拟南芥（Arabidopsisthaliana）中，已鉴定出15个OSCA家族成员，这些成员具有高度保守的氨基酸序列，并携带与植物抗逆性相关的DUF221结构域[45]。（剩余12208字）