半夏ARF转录因子家族的全基因组鉴定及生物信息学分析

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中图分类号：S567.2 文献标识码：A 文章编号：1000-4440（2025）06-1095-12

Abstract：Pineliaternata isanimportant medicinal plant.During itsgrowth，itisextremelysensitivetolightand temperature，andpronetothephenomenonofseedlingcollapse，whichleads toareduction inyield.Inthisstudy，basedon thegenomicdataof PinelliaternatareleasedbytheChinaNationalCenterfor Bioinformation，25membersofthePtARF familywereidentifiedanddividedintofoursub-familiesthroughphylogeneticanalysis.Theresultsofproteinphysicochemical propertyanalysis showedthatallPARFs inPinellaternatawere hydrophilicandunstableproteins，and mostPtARFs werelocatedinthecellnucleus.Thepredictionoftheprotein tertiarystructureindicatedthatthe tertiarystructuresof  PtARF family members with close genetic relationships werehighlysimilar，suggestingpotential functional redundancy among some PtARF proteins. The resultsof phylogenetic analysisandcollinearityanalysisshowed that the ARF genesofPinellia ternataand ricewerecloselyrelated.In the promoter regions of the ARF family genes in Pinelliaternata，thecis-actingelementswiththelargest number were those related to biotic and abiotic stress responses，followed byplant hormone-responsive elements，

andtheelementsrelatedtoplant growthanddevelopmentresponsesweretheleast innumber.Thisresultindicated that PtARFsmay be widely involved in theresponseof Pinellia ternata to stress.Further research results showed that PtARF couldrespond tolow-lightstressandhigh-temperaturestress，andmaybeinvolvedintheregulationofseedlingcollpseand tuberdevelopmentinPinelliaternata.Thisstudyprovidesanimportanttheoreticalbasisforstre-resistantbredingandoptimization ofcultivation techniquesofPinellia ternata.

Key Words： Pinellia ternate；ARF transcription factor family；sprout tumble；bioinformatics

半夏（Pinelliaternate）是天南星科半夏属多年生草本植物，其块茎含有多种生物活性成分，包括生物碱、有机酸、多糖、蛋白质和核苷等[1]。（剩余15833字）