菊花脑CnNAC83基因克隆及其表达分析

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【Abstract】Using stemand leaf tissues of Chrysanthemum nankingense，we clone the NAC transcription factor gene CnNAC83and perform bioinformaticsanalysis along with expressonprofiling underPEG-6Ooo-simulated drought stress. ThecodingsequenceofCnNAC83is786bpinlength，encoding261aminoacids，withaconservedNAMdomainatits N-terminus，anditssubcellularlocalizationispredictedtobethenucleus.Physicochemical propertyanalysisshowthathe isoelectricpointofCnNAC83is9.14，andagrandaverageofhydropathicity（GRAVY）score-0.725，indicatingitisahdro philicprotein.Phylogeneticanalysisshow that CnNAC83 ismostcloselyrelated toAaNAC83fromArtemisiaannua，while moredistantlyrelatedtoPgNAC83fromPunicagranatumandAtNAC83fromArabidopsis thaliana.RT-qPCRanalysis show thatdrought stresscan induceCnNAC83 expression.Theseresults indicate that CnNAC83 playsapositiveregulatoryrole in droughtresistanceinChrysanthemumnankingense，whichprovidesabasisforthestudyof thebiological functionof CnNAC83.

【KeyWords]Chrysanthemumnankingense;genecloning;NACtranscriptionfactor;droughtstress; expresionanalysis

[中图分类号］Q781 [文献标识码]A [文章编号]1674-3229（2025）02-0107-08

0 引言

在植物的生命周期中，保持基因按照特定时空顺序进行表达对于维持其发育进程至关重要，而在这一过程中转录因子扮演着不可或缺的基础角色。（剩余9991字）