多年生黑麦草对镉和砷复合胁迫的响应及酶学机理

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中图分类号：S53;X173;S543.6 文献标志码：A 文章编号：2095-6819（2025）05-1354-12 doi：10.13254/j.jare.2024.0450

Response and enzymatic mechanisms of Lolium perenne L.to combined cadmium and arsenic stress

XIE Xiaoyan1，DENGJiangdi²，QIHuacai1，LIANGXinran1，WANGLei1，LIYuan1*，LI Zuran3* （1.CollgeofsosdEviont，uagiclalUivesityKuingi;.Fcultyfialid Technology，YanAgiculturalUiversityKunming0lhina;3olgefanscapendHorticulture，Yuanlal University，Kunming ， China）

Abstract：Theimpactofsingletoxictraceelementsonforagegrasshasreceivedwidespreadatention，butstudiesontheresponseof LoliumperenneL.toCd-Ascombinedtressaresarce.ToinvetigateteftsofCd-Asobindstressonthegrowhracteistics photosynthesis，enzyme mechanism and transporter of perennial ryegrass，in this study， L* perenne‘Nicaragua’and L. perenne‘Venus' were grown in a greenhouse with the following treatment groups ： control group（Cd 0mg⋅kg-1 ，As 0mg⋅kg-1 ）， Cd3As60（Cd 3 mg·kg-1 ，As 60mg⋅kg-1 ），Cd3As120（Cd 3mg⋅kg-1 ，As 120mg⋅kg-1 ），andCd3As180（Cd 3mg⋅kg-1 ，As 180mg⋅kg-1 ）.Theaccumulation characteristics ofCd and Asin L* perenne，as wellas their enzymatic mechanisms，were analyzed.The results showed that compared with Cd3As60，underhigh-concentrationCd-Asstress，thecontentofMDAintheshootsof‘icaragua’and‘Venus’significantlyinceased （ 12.93%-24.73% and 16.02%-29.98% ），the activity of antioxidant system was enhanced.The chloroplast stroma thylakoid arrangement wasdisorderedlalldfdsulidosiscd28.32% and 1.75%-7.20% ）and reduced biomass（ 36.22%-54.56% and 2.96%-17.82% ）.The content of Lsil protein in the shoot and root of‘Nicaragua’significantly increased by （12.23%-20.26% ）and （13.39%-15.64% ），which increased the proportion of As in the soluble fractions（ 2%-11% and 1%-12% ），thereby the As toxicity to cells was alleviated.Compared with Cd3As6O，under highconcentrationCdrssiteasfslstttiitto‘Veadtelleveld， the contentsof GSH，PCs，NPT，CAXandNrampreduced，whichdecreasedtheaccumulationof Cdinshootsandroots（8. 96%-13.18% and 9.47%-18.59% ）and its proportion in soluble fractions （ 1%-9% and 0%-2% ）. Cd-As stress could induce oxidative stress and cell membranestructuredamage，decreasethecontentofthiolsubstancesandtransportproteins，esultingineducedacumulationofCdand As in L. perenne.

Keywords：Cd-As combined stress; photosynthesis; enzymatic mechanism; transporter

镉（Cd）和碑（As）均被国际癌症研究机构列为I级致癌物1，其主要通过金属冶炼、矿山开采和污水灌溉等工农业活动在土壤中不断积累，导致农作物严重减产。（剩余20385字）