意大利黑麦草表面微生物对环境胁迫的响应

打开文本图片集

中图分类号：S543.6 文献标识码：A 文章编号：1007-0435（2025）09-2873-07

doi：10.11733/j.issn.1007-0435.2025.09.012

Responsesof Microbeson the SurfacesofItalian Ryegrass to Environmental Stress

TANG Guo-jian1， YANG Jin-mei¹，MENG Yuan-yan²， SONG Qiong-mei²， SUN Ting²， WANG Yi2 ， WU Dan2*， XU Liu-xing2* （1.Liupanshui Normal University，Liupanshui，Guizhou Province 553oO4，China; 2.Zhaotong University，Zhaotong，Yunnan Province 657ooo，China）

Abstract：To investigate the responses of microbes on the surfaces of Italian ryegrass to environmental stress， this studycompared the efects offour drought stresslevels（control，mild，moderate，and severe），three ambient relative humidity（RH） （50%RH ， 70%RH ，and 90%RH） ），two temperatures （ 15∘C and 25∘C ），and three photoperiods （ 12h ， 16h ，and 22h ） on the count of microbes on the surfaces of Italian ryegrass. The results indicated that thecount of lactic acid bacteria on the surfaces of Italian ryegrassshowedafirstly significant decrease and then an increase （P<0.05 ，while the count of mold and yeast showed a significant decrease （ P< 0.05）with the extension of drought time.In contrast，increasing ambient humidity significantly increased the count of lactic acid bacteria on the surfaces of Italian ryegrass，while also increasing the abundance of other microrganisms（excluding yeast）．Except for lactic acid bacteria，the microbial quantities on the surfaces of Italian ryegrass planted at 15∘C and 25∘C were significantly different （ .P<0.05; .The count of lactic acid bacteriaon the surfaces of Italian ryegrass showed a trend offirst significantly increasingand then decreasing with the extension of the photoperiod，but other microbes were not significant change. In summary， 90%RH ，and a 16h photoperiod were the most favorable condition for the survival and reproduction of lactic acid bacteria，and mild drought stress reduced the count of mold and yeast on the surfaces of Italian ryegrass.

Key words：Italian ryegrass;Lactic acid bacteria;Drought stress;Photoperiods;Temperature and humidity

植物表面微生物群落构成了一个高度复杂且动态变化的生态系统1，其构成与分布不仅深受宿主植物种类的影响[，同时也显著受制于多重环境因素，包括UV-B辐射强度、生境温度、相对湿度（Relativehumidity，RH）以及土壤养分可利用性等[3-5]。（剩余11164字）