图们江流域部分市县蜱传病原体分子流行病学及蜱体内菌群调查

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中图分类号：S852.746 文献标志码：A 文章编号：0366-6964（2025）06-2927-10

Molecular Epidemiology of Tick Borne Pathogens and Investigation of Tick Borne Microbiota inSome Citiesand Countiesinthe Tumen RiverBasin

YIN Guolu， YU Tian，WANG Jinqi， XU Ying，LIU Zhe，GAO Xu，YU Longzheng， JI Shengwei，XUE Shujiang＊，JIN Dongchun* （Agricultural College of Yanbian University，Yanji 133oo2，China）

Abstract： In order to systematically understand the tick-borne pathogens in the Tumen River Basin and to investigate the tick flora， the molecular epidemiology of tick-borne pathogens of Haemaph ysalis and Dermacentor as wellas the tick flora in the Tumen River Basin were investigated in the experiment. Dermacentor siluarum， Haemaphysalis japonica， Haemaphysalis concinna were collcted at 5 sampling points in 3 cities and counties in the Tumen River basin. Tickborne bacteria were investigated using high-throughput sequencing technology，and 147 ticks were analysed by second-generation sequencing of the V3-V4 variable region of the 16S gene of tick-borne bacteria. PCR was performed on 442 ticks carrying spotted fever groups Rickettsia ， Anaplasma and Babesia. The results of the study showed that analyses of high-throughput sequencing results revealed the presence of at least two tick-borne pathogens and six potentially pathogenic genera with some pathogenic strains within the genus. There were differences in microflora carried by ticks of different genera and sexes in three cities and counties. One type of Rickettsia was detected in ticks in the region： Rickettsia raoultti；One type of Anaplasma ： Anaplasma capra ; One species of Babesia ： Babesia caballi. The obtained results provide basic data for tick borne pathogens and microbial communities in the region， and provide data support for the prevention and control of ticks and tick borne diseases.

Keywords： Tumen river basin； ticks；tick borne pathogens； tick microbiota;investigation∗ Corresponding authors：XUE Shujiang，E-mail： sjxue $＼textcircled { ＼alpha }$ ybu.edu.cn；JIN Dongchun，E-mail：jindongchun@ybu. edu. cn

蜱是一种吸血节肢动物，可在哺乳类、鸟类、爬行类和两栖类脊椎动物的体表寄生，仅次于蚊的虫媒病原传播媒介[1]。（剩余12828字）