紧密连接蛋白CLDN4促进猪流行性腹泻病毒感染

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中图分类号：S852.659.6 文献标志码：A 文章编号：0366-6964（2025）06-2826-10

Tight Junction Protein CLDN4 Promotes Porcine Epidemic Diarrhea Virus Infection

LI Chengcheng1，² ， ZHAO Yongxiang²， CAO Qiuxia²， SONG Xu2 ， LI Yupeng²， FAN Baochao²，GUO Rongli²，XU Yefen1*，LI Bin1，2 * （1. Xizang Key Laboratory of Hydatidosis Control， Ministry of Agriculture and Rural Area， College of Animal Science， Xizang Agricultural and Animal Husbandry University ， Linzhi 86oooo， China ; 2. Institute of Veterinary Medicine， Jiangsu Academy of Agricultural Sciences，Nanjing 2lool4，China）

Abstract： Tight junction proteins play a crucial role in various viral infections，with tight junction protein 4（Claudin-4，CLDN4） being a major member of this family. The role of CLDN4 in porcine epidemic diarrhea virus （PEDV） infection remains unclear. To explore the impact of PEDV infection on CLDN4 expression and the role of CLDN4 in PEDV infection，we performed quantitative PCR （qPCR） and Western blot to assess the transcriptional and protein expression levels of CLDN4 follwing PEDV infection. Additionally，a eukaryotic expression plasmid for the CLDN4 gene was constructed，and specific interference RNA targeting CLDN4 was designed. The effects of overexpresson and knockdown of CLDN4 on PEDV replication were analyzed using qPCR， Western blot，immunofluorescence assay （IFA），and PEDV viral titer measurements. Finally， we examined the effects of overexpression of CLDN4 on the adhesion and invasion phases of PEDV infection. Results showed that PEDV infection upregulates CLDN4 transcription and protein levels，while overexpression of CLDN4 significantly promotes PEDV replication， and interference with CLDN4 expression inhibits PEDV replication. Moreover，CLDN4 facilitates PEDV adhesion and invasion. This study suggests that CLDN4 plays a role in promoting PEDV infection and replication， providing a reference for investigating CLDN4 function and elucidating PEDV replication mechanisms.

Keywords： CLDN4;porcine epidemic diarrhea virus;promote;infection ∗ Corresponding authors：LI Bin，E-mail： libinana 126.com；XU Yefen，E-mail： xzlzxyf 163.com

猪流行性腹泻病毒（porcineepidemicdiarrheavirus，PEDV）是一种猪肠道冠状病毒，为有囊膜的单股正链RNA 病毒[1]。（剩余16259字）