猪防御素-1的原核表达及抑菌效应分析

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中图分类号：S859.797 文献标志码：A 文章编号：0366-6964（2025）06-2836-11

Abstract： The aim of this study was to investigate the antibacterial activity in vivo and in vitro of PBD-l over-expressed by prokaryotes，and to provide material and theoretical basis for the treatment of clinical mastitis. In this study，prokaryotic expression vectors of PBD-1 were constructed，the induced expresson conditions were optimized，the minimum inhibitory concentration （MIC）was determined after over-expression and purification，and the bactericidal dynamic curve was drawn. The mouse model of mastitis was established with E .coli 13-1 and S .aureus 4-1， and the intramammary perfusion therapy was performed with different concentrations of PBD-1， and the therapeutic effect was observed through the pathological changes of breast tissue and its bacterial load. The results showed that the prokaryotic expression vector of PBD-1 was successfully constructed，the recombinant PBD-1 was soluble and yielded of 328.6mg⋅mL-1 . The recombinant PBD-1 had antibacterial effect on E . coli l3-1，S. aureus 4-1 and S . epidermidis 25- 1，and the MIC was 82.15μg⋅mL-1 in vitro. The results of intramammary perfusion test showed that PBD-1 with 1×MIC and 2×MIC had a certain therapeutic effect on S . aureus 4-1 and E . coli 13-l mouse mastitis models. Prokaryotic expression of recombinant PBD-1 has inhibitory effects on all three detected bacteria in vitro，and has therapeutic effects on S.aureus 4-1 and E . coli 13-1 mastitis models.

Keywords： prokaryotic expression; mastitis; PBD-1; antibacterial activity in vitro ; animal model ∗ Corresponding authors： JIANG Yanfen，E-mail： jyfl111 nwafu. edu. cn；ZHENG Xueli， E-mail： zhengxueli@nwafu. edu. cn

抗菌肽（antimicrobialpeptides，AMPs）是生物机体自身合成的一类小分子多肽，具有抗微生物、抗肿瘤活性，在先天免疫系统中发挥着关键作用[]。（剩余17214字）