高产油小球藻的超声波诱变育种

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中图分类号：Q689;TS222 文献标志码：A 文章编号：1002-4026（2025）04-0078-08

Abstract：Toenhance the microalgae lipidcontent，Chlorela sp.wassubjectedtoutrasonic mutagenesis.Mutagenesis was performed for 20 minutes under conditions 40kHz 100W ，resulting in the cultivation 10 mutant strains （M1- M10）.Further screening ledtotheselection three stable high-lipid-producing strains，namelyM2，M5，M7.These strains were analyzed todetermine their biomass concentrationlipid，chlorophyll， protein massconcentration percentage.The results indicated that the biomass mass concentrations M2，M5， M7 were 0.384，0.379， 0.443g/L respectively. The lipid yields M2，M5， M7 were 14.70，13.34， 25.11mg/L ，respectively，all which were higher thanthat the wildtype（WT）strain，whichhadarecorded yield 9.38 mg/L.Comparedto the WT，the chlorophyllyield the mutant strains increased by 14.41% ， 3.01% ， 10.24% ，respectively，while the protein content the mutant strains increased by 7.30% ， 10.52% ， 13.66% ，respectively. This study is great significance to the industrialized production biodiesel.

Keywords ：Chlorella sp.；ultrasound；biomass；lipid；chlorophyll；protein

化石燃料的燃烧导致了严重的大气污染、温室效应等环境问题，对全球的可持续发展构成重大的挑战[1]，寻找化石能源的替代品越发迫切[2-3]。（剩余10035字）