领鞭毛虫Salpingoecarosetta的纤毛组装研究

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中图分类号：Q28 文献标志码：A 文章编号：1002-4026（2025）04-0039-07

开放科学（资源服务）标志码（OSID）

Abstract ：To investigate the mechanismofteciliaassembly inchoanoflagellates，thisstudyfocusedonacolony-forming choanoflagellte，Salpingoecarosetta.Forthefirsttime，theciiaofSalpingoecarosettaweredisassembledviamechanical shearing，anda LiCl（lithiumchloride）-inducedciliaelongationexperimentwasconductedtoinvestigatemechanisms underlyingthedisassembly，regeneration，andassemblyoftheciliaofSalpingoecarosett.IntheLiCl-induced cilielongationexperiment，afinalmolarityof250mmol/Lwasused.Intheciliadisassemblyandregenerationexperiments， the mecanical shearingconditions were determined.Experimentalresults showthattheciliaof Salpingoecarosetta were restored to their original lengths after a mechanical force was applied on them for 120min ；moreover，LiCl-induceda ciliaelongation by more than 60% after 180min of LiCl treatment.Compared with the cold-stress-induced disassembly of the cilia of Salpingoeca roseta，the mechanical shearing methodwasfaster，moreeficient，andsimplerwith higher repeatability.Thisstudyprovidesafundamentalmethodforinvestigatingthemolecular mechanismof theciliaassemblyin Salpingoecarosetta.

Keywords：Salpingoecarosett;LiCl-inducedciliaelongation；ciliaregeneration；ciliaassembly；mechanical shearing

领鞭毛虫是现存与动物最接近的单细胞姐妹群[1-3]，而群体形成性领鞭毛虫（Salpingoeca rosetta）是一种生活在海洋里的领鞭毛虫，文献报道它的多细胞生活史可能揭示了动物多细胞的起源[47]。（剩余11302字）