大姜根腐病生防木霉菌株的分离与鉴定

打开文本图片集

中图分类号：S476.1;Q939.9 文献标志码：A 文章编号：1002-4026（2025）04-0056-11

Abstract：Gingerrootrotisasoil-borne diseaseinginger planting，which ismainlycausedbyFusarium spp.andcan induceserious reductionoreven extinctionof ginger yield.Thirty-fourTrichoderma strains withantifungal activity were isolated from thesoilcolected from representative ginger planting areas in Shandong province.Basedontheir antibiotic properties，antagonisticcoeficients，andsporeproductioncapabies，three highlyeffectiveTrichodermastrains were selectedfor their antagonismagainst Fusarium oxysporum f.sp.zingiberi（Foz）：TW20111，TW20321，and TW20323.Morphological observationsandmolecular biological identications clasifiedstrainTW20l11asTrichodermaatrovirideand strainsTW20321andTW20323asTrichodermaharzianum.Theeficacyof these threeTrichodermastrainsincontroling gingerroot rot wasassessed under greenhouse andfieldconditions.Under greenhouseconditions，T.harzianumTW20321 showed the highest control efficacy of 86.33% ，significantly promoted ginger plant height，and moderately increased ginger yieldcomparedtocarbendazimtreatment.Fieldexperimentsrevealedthatthecontroleficacyofanytwocombined Trichodermastrains wassignificantly higherthanthatofasinglestrain，withthecombinationofTW20l1andTW20321 achieving the highest control efficacy of 68.90% . This combination also resulted in the greatest increases in plant height and single plant yield，which rose by 19.31% and 27.43% ，respectively，compared to the control group.This study provided a basis for the development of new bio-pesticides for the effective control of ginger root rot.

Key words ： Trichoderma spp.； ginger root rot； Fusarium oxysporum f. sp. zingiberi； biocontrol

大姜（Zingiber officinale Rosc.）又称生姜，属姜科姜属，为多年生单子叶草本植物，在我国作为一年生经济作物栽培。（剩余13455字）