罗汉果ISSR-PCR反应体系优化及引物筛选

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关键词罗汉果;ISSR；体系优化；引物筛选中图分类号 S567 文献标识码A文章编号 0517-6611（2025）21-0100-05doi ：10.3969/j .issn.0517-6611.2025.21.018

AbstractObetie]TestablishaneicentadstableISSR-PCRreactionstemaproprateforSraitgosenori.MethdOrnal test design L16（45） was used. The template DNA，primer， Mg2+ ，dNTPs and Taq enzyme that affect the ISSR-PCR reaction system were optimized，andtheptialISSR-PCRsystemforraitiagosenori wasdetermined.ResultTheexperimentalresultssoedthatina 20μL reaction volume，the optimal ISSR-PCR reaction system conditions for Siraitia grosvenorii were 10× Buffer 2.0μL，Mg2+ 1.5 mmol/L （1.2μL ）， dNTPs 0.3 mmol/L （ 2.4μL ），Taq enzyme 1.0U ，primer 0.4μLmol/L （ 0.8μL ，and template DNA 75 ng（ 1.5μL ）.On the premise of optimalreactiooios，salprirsreeexperetsdprirsitaadgularndseleted Finaly，teosteficitaealingtmperatureofpresassletedCoclusion]hissudyproviddaeferecfortesudyofte close relationship between varieties，genetic resources and biological diversity of Siraitia grosvenori.

Key wordsSiraitia grosvenorii; ISSR;System optimization;Primer screening

罗汉果［Siraitia grosvenorii（Swingle.）C.Jeffrey]为葫芦科药用植物，药用部位为干燥果实。（剩余7976字）