黄沙鳖败血症病原菌的分离鉴定及毒力基因研究

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关键词黄沙鳖；嗜水气单胞菌；迟缓爱德华氏菌；分离与鉴定；毒力基因

中图分类号S947.1文献标识码A

文章编号 0517-6611（2025）21-0077-05

doi：10.3969/j.issn.0517-6611.2025.21.015

AbstractObjective]InordertoidenifythecauseofmassdeathofTuogxsinensis inanquaculturefarinGuangxi，andtouderstand thevirulecegscidbypathogenicbacteria.MethdBacteaeresolatedfrotevisceraltisusofuogsinesisitsisby routinemetods，ndtrpysiologicalandohemicalaracteristicsndmleularloedentifdeathogecityfs wasdeterminedbyrtificialinfectiontestofsinglestrainandmixedstrainThevirulencegenesofpathogenicstrainwredetectedbyPCR. [Result]TwodinantstrainsYOandYO2wereisolatedfromkidneytisueofTuogxsinesistrainYOwasAeromonashdoila， and the homology of 16S rRNA gene sequence with Aeromonas hydrophila strain M83 was 98.21% . Strain YLO2 was Edwardsiella tarda，and the 16S rRNA gene sequence had 99. 12% homology with Edwardsiella tarda strain S4.The results of artificial infection showed that strains YL01，YO2didacteralolutidropathogenicityemposfseasdT.nsisirtfalifetioe asthosfnatualifectioderomoasdroidEddselldesolaedfrothsudgasofrtifllfd Truogx sinensis. The virulence genotype of strain YLO1 was hly+Aer-Alt+Act-ahal+ahp+ ，and the virulence genotype of strain YLO2 was citC+fi 1 （20 mA+gadB+mukF+esrB-katB- .[Conclusion] Aeromonas hydrophila and Edwardia tarda are the pathogenic bacteria causing the death of Truogx sinensis.heidifetiontestsulsftetoacteiaalsdcateatteifectioiuatioofuogsiesisihssisso plex than before，and attention needs to be paid to disease prevention and control.

KeyWordsTruogx sinensis;Aeromonas hydrophila;Edwardsiella tarda;Isolationand identification; Virulence gene

黄沙鳖（Truogxsinensis）是广西地方名优品种，分布于广西壮族自治区內的邕江、西江、左江、右江、郁江等流域，尤以广西贵港市内的浔江、郁江分布最多。（剩余8009字）