酵母钴的制备、表征及其在鱼抗应激中的应用

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分类号：S963 文献标志码：A 文章编号：1008-0864（2025）09-0240-10

PreparationandCharacterizationofYeastCobaltand Its Application in Fish StressResistance

SHI Kerou1，WANG Nan，LUAN Yinyin1，LI Yanqing'，ZHANG Zhen¹，YAO Yuanyuan 1 RAN Chao1，DING Qianwen1，YANG Yalin1*， ZHOU Zhigangl.，2* （1.InstituteofFeedResearchofChineseAcademyofAgriculturalSciences，Beijing1Oo81，China；2.BiotechnologyRsearch Institute，ChineseAcademyofAgriculturalSciences，Beijing1Ooo81，China）

Abstract：Cobalt（Co）isan important essntial traceelementforaquaticanimalsand iscriticalfor maintaining their physiological and metabolic functions.Probiotics canconvert inorganiccobalt into organic cobalt forms with less toxicityand higher bioavailability，providing dual benefitsofcobaltandprobiotics.YeastR wasscreened to improve cobalt-enrichedabilityfromtheprobioticsrecommendedbytheEuropean UnionQualificationfortheSafetyof Microbial Strains（QPS），which had high toleranceand enrichmentcapacity for cobalt chloride.Theoptimal cobalt enrichment conditionswasdetermined asinitial pH5.5，incubation temperature 30∘C ，incubation time 76h ，and the addition of1.5 g⋅L-1 cobalt chloride for 4 h ，resulting in total cobalt content of 3.78 g⋅kg-1 .Characterization tools confirmed the possible presence of spherical cobalt on the surface of cobalt-enriched yeast（R-Co）nanoparticles.In the zebrafish test，the addition of 0.166 7 mg kg-1 R-Co had no effect on growth performance and improved survival rate under ammonia-nitrogen stress and cold stress compared with the addition of 0.1667mg*kg-1 inorganic cobalt，suggesting thatyeast Risaneficientcobalt-enriched strain with thepotential of replacing inorganiccobalt for promising applications inimproving the health and resilience ofaquatic animals.

Keywords：probiotic yeast；trace element cobalt；characterization；zebrafish；stress resistance

钴是构成维生素 B12 的关键成分，占比大约为 4.5% ，多数动物都依赖钴来完成维生素 B12 的合成过程-2]。（剩余15263字）