棉花VOZ基因家族鉴定及GhVOZ1耐盐功能研究

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中图分类号：S562 文献标志码：A 文章编号：1008-0864（2025）09-0058-11

Genome-wideIdentificationofVOZGenesFamily in Cotton and Study on Salt Tolerance Function of GhVOZ1

JIA Hao 1 ，WANG Hongzhe²，SUN Zhengwen1，GU Qishen1，ZHANG Dongmei 1 ，WANG Xingyi 1 ZHANG Yan1，LU Huaiyu²，MA Zhiying1，WANG Xingfen1*

（1.State KeyLaboratoryof North ChinaCropImprovementandRegulation，KeyLaboratoryforCropGermplasmResourcesof

Hebei，Collge of Agronomy，Hebei Agricultural University，Hebei Baoding O71Ooo，China; 2. Xinjiang Guoxin Sd Industry Co.，Ltd.，Xinjing Luntai 84160o，China）

Abstract：The vascular plant one-zinc finger protein（VOZ）is a transcription factor specific to higher plants，which is involved inregulatingplant growthanddevelopment，aswellasresponding tobioticandabioticstresss.Toidentifythe VOZ gen familyofcotonandanalyze thefunctionof the GhVOZ1geneundersalt stress，theVOZ familyindiploid and tetraploidcottn species was analyzed.The results showed that diploid and tetraploid cotton species contained 3and 8 VOZ members，respectively，and the structures of most VOZs genes in Gossypium hirsutum and G. barbadense were highly conserved.GaVOZ1，GrVOZ1，GhVOZ1，GhVOZ5，GbVOZ1andGbVOZ6 clustered together with several salttolerant genes，indicating that this group of VOZs had similar functions.The expression of GhVOZs was significantly upregulated undersalt stressfor12h.Among them，the expressionlevelsof GhVOZ1and GhVOZ5 were upregulated by nearly7times compared with the watercontrol，suggesting that these2 genes were importantcandidate genes involved in the response tosalt stress.GhVOZ1 was localized in cytoplasm，anditsoverexpression in Arabidopsis thaliana could significantly enhance thesalt toleranceof theplants.Aboveresultsprovidedreferencefordeeperunderstanding of the functionof cotton VOZ genes undersalt stressandoffer new gene resources for molecular improvementofsalt tolerance.

Keywords：cotton；vascular plant one-zinc-finger protein；Gh VOZ1；salt stress tolerance

棉花能够提供天然可再生型纺织原材料，具有重要的经济价值，同时也是开发利用盐碱地的先锋作物。（剩余12965字）