异源表达垂序商陆PaMT3-1基因提高拟南芥盐和渗透胁迫抗性

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HeterologousExpressionof PaMT3-1 Gene from Phytolacca americanaEnhancesSaltandOsmoticStressTolerancein Arabidopsisthaliana

Yueriyeti Sali，NuerbiyeYisimayi，AliyaWaili，CHENLingna*，CHENYongkun* （XinjiangKeyLaboratoryofSpecialSpeciesConservationandRegulatoryBiology，LaboratoryofPlantStressBiologyinAridLand， SchoolofLifeSciences，XinjiangNormalUniversity，Urumqi83oo54，China）

Abstract：Metallothionein（MT）isacysteine-rich，low molecular weight metal binding protein，which iscrucial for regulating metal homeostasis and enhancing tolerance toabiotic stressThe cadmium-resistant metalothionein gene PaMT3-1 from Phytolacca americana was introduced into Arabidopsis，andtherot length，fresh weight， physiological and biochemical indexes of transgenic strains and wild type were determined under 100 mmol·L-1 NaCl and 200 mmol·L-1 mannitol stress.The results showed that the expression of PaMT3-1 in transgenic Arabidopsis plants increased resistance tosalt and osmotic stress.Compared to the wild-type，the transgenic lines exhibited significantlygreaterrootlength，freshweight，andchlorophyllcontent.Therootlengthsoftransgenic linesOE1and OE3 was more than twice that of wild-type line when treated by 1OO mmol·L-1 NaCl and 200 mmol·L-1 mannitol，and the transgenic lines exhibitedanotable increase in prolinecontent and activities of peroxidase（POD），superoxide dismutase （SOD）and catalase（CAT） under stress conditions.Particularly，the POD activity in OE1 treated by 200 mmol·L-1 mannitol was 9.85 times higher than that of the control.Above results indicated the transgenic lines had enhancement of osmoticadjustmentabilityand antioxidant enzyme activities.MTgene responded to abiotic stress in plants through various mechanisms，and could be used asauniversal gene resource for plant resistance to various abiotic stresses，which provided a reference for molecular improvement of crop resistance. Keywords：metallothionein;antioxidation;salt stress;osmotic stress

金属硫蛋白（metallothionein，MT）是一类富含半胱氨酸（cysteine，Cys）的低分子量结合蛋白，普遍存在于高等动植物及微生物中]。（剩余12596字）