脂肪酶产生菌的分离鉴定及产酶条件优化

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中图分类号：TQ925.6 文献标识码：A 文章编号：2095-5774（2025）02-0155-07

Isolation and identification of lipase producing bacteria and optimization of enzyme-producing conditions

Li Guofeng 1，2 +， Zhou Shiwen 1，2 +， Cheng Junqiu 1，2 †，Li Zunwen 1，3 ， Jiang Ran4， HongYingqi ³，XuQiaoling5，YeWei1，3*

（1.Key Laboratory of Crop Genetic Improvement and Innovative Utilization in Fujian Province，Sanming， Fujian 365509，China； 2.Fujian Agriculture and Forestry University，Fuzhou，Fujian 35ooO2， China; 3.Institute of Medicinal Plants，Sanming Academyof Agricultural Sciences，Sanming，Fujian 3655o9，China; 4.FuJian WeLike Pharma Co.，Ltd， Sanming，Fujian 365509，China; 5.Gansu Third People'sHospital，Lanzhou， Gansu 730O20， China）

Abstract：【Objective】The purposeof this experiment is to screen lipase producing bacteriaand studytheir enzyme producing ability.【Method】Thesoil samples which had been contaminated byoil pollution foralong time，wereenriched and cultured，and the strains were isolatedandscreened by Rhodamine Bplate method.The shaking tableculture with peanutoilas theonlycarbonsourcewas used to explore the enzyme production parametersof theselected strains.The lipase production conditions of each strain weresystematicallyoptimized.【Result】Three strains with high enzyme production ability were obtainedusing Rhodamine B plate methodand namedasLN2，Bk3andBd4.Throughbymorphologicaland16S rRNAmolecular identification，LN2wasPseudomonasaeruginosa，Bk3was Burkholderiavietnamiensisand Bd4 was Acinetobacter soil.After 48 hours of cultivation，the optimal temperature for the three strains is 30～40% ，and the optimal culture pH is 7.O .Through comprehensive screening，the enzyme activity of LN2 was found to be 1.25U/mL at 30% and pH 7.0; the enzyme activity of Bk3 was O.85 U/mL at 40% and pH 7.O; and the enzyme activity of Bd4 was 3.13 U/mL at 30% and pH7.0.【Conclusion】The three lipaseproducing bacteria showed excellent potential in lipaseproduction，especially Bd4， which provided candidate strain materials for the industrial application of lipase.

Keywords：lipase; Pseudomonas aeruginosa; Burkholderia vietnamiensis; Acinetobacter soil; optimal temperature

脂肪酶是自然界中普遍存在的水解酶类，能促进脂肪分解为甘油与脂肪酸，且在非水相体系中展现出优异的催化活性[1-2]。（剩余10091字）