波罗蜜花粉生活力测定与花粉萌发培养基的优化

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中图分类号：S667.8 文献标识码：A 文章编号：2095-5774（2025）02-0148-07

Determination of pollen viability and optimization of pollen germination medium in Artocarpus heterophyllusLam.

He Hui， Wu Weihao，Luo Zhongjun，Tan Qiaoru， Feng Feng* （College of Coastal Agricultural Sciences，Guangdong Ocean University，Zhanjiang， Guangdong ，China）

Abstract：【Objective】This studyaimed to screentheoptimalmedium formulationfor in vitro germinationofArtrocarpus heterophylusLam.pollenandtoevaluate theapplicabilityofrapid staining methodfordetectingpollenviability.【Method] Pollen of‘Siji’A.heterophylus was used as experimental material to measure pollen germinationrateandoptimize polln culture medium composition by single factor test and orthogonal test.【Result】The effects of sucrose， H3BO3 ， CaCl2 and MgS04 on pollen germination rate were significant， with CaCl2 ranking first，followed by sucrose， H3BO3 ，and MgSO4 . The optimummedium formulawas 5% sucros Ω3+150mg/LH3BO3+100mg/LCaCl2+150mg/LMgS04 ，and the germination rate was72.39%，which was significantly higher than other treatments.The optimum germination temperature was 27% ： Both theMTTstaining method（10 min staining，62.99%viability）andthe TTC staining method（45 min staining， 78.58% （20 viability）couldbeusedforrapid detectionof pollen viability.The MTTstaining method was more eficientand had better contrast ratio while the magenta acetate and I2 -KI methods were not applicable.【Conclusion】The results provide a theoreticalbasisandpracticalsupportforpollenviabilitydeterminationandartificialpolination technology inA. heterophyllus.crossbreeding.

Keywords：Artocarpus heterophylus；polen；viability；germination；medium；MT（2，5-diphenyl monotetrazolium bromide）staining; TTC（triphenyl tetrazolium chloride） staining

波罗蜜（ArtocarpusheterophyllusLam.）为桑科（Moraceae）波罗蜜属（Artocarpus）典型的热带果树，别名木菠萝、树菠萝等，英文名Jackfruit[1]原产于印度西高正山脉的雨林，现世界各地广泛栽培，主要分布于南亚、东南亚和澳大利亚等地[2。（剩余10077字）