近红外次氯酸荧光探针的构建及其类风湿性关节炎成像研究

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关键词：生物成像；荧光探针；次氯酸；真实水样；类风湿性关节炎中图分类号：0482.31 文献标识码：ADOI：10.37188/CJL.20250050 CSTR：32170.14.CJL.20250050

Construction of Near-infrared Hypochlorous Acid Fluorescent Probe and Its Imaging in Rheumatoid Arthritis

HEJingl†，YANG Xinyi1+，SHANG Zhuye"，ZHANG Zhiqiang1，WANG Yanshai²，MENGQingtac ⋅1* （1.SchoolofChemical Engineering，，UniversityofScienceandTechnologyLiaoning，Anshan114051，China; 2.SchoolofChemical Engineering，DalianUniversityofTechnology，Dalian116024，China） *CorrespondingAuthors，E-mail：shangzhuye@ustl.edu.cn;qtmeng@ustl.edu.cn

Abstract：Elevated levelsof hypochlorousacid（HOCl）may beoneof the hallmarks ofchronic inflammatory joint disease.Therefore，thedevelopmentofeffective methods forrapidandaccurate detectionormonitoringofHOClisimportant forfurtherunderstanding theroleofHOClinrheumatoidarthritis（RA）.Herein，anear-infrared（NIR）fluorescentprobe （HN）forthe detectionand monitoring of HOCl was reported.In the presenceof HOCl，the phenolic hydroxylgroupof HN is oxidized to produce quinone compounds，resulting inquenching of its NIR fluorescence.Probe HN has several advantages such as long emission wavelength （ λem =660 nm），large Stokes shift（2OO nm），high selectivity and sensitivity （LODis 54.8 nmol/L），and widepHapplicabilityrange（4.5-10.5）.The“naked-eye”detectionof HOClinreal water samples has beenrealizedusing the probeHN-basedtest strip.Inaddition，the probe has beensuccessfullyused forfluorescence imaging of exogenous and endogenous HOClin living zebrafish and mice.Most importantly，the probe HN has shown remarkable efectiveness in monitoring HOCl-mediated responses toRA therapy.Thus，HN provides anovelapproach to further understand the role of HOClinRAand evaluate the effcacy of drug therapy for RA.

ey words：bioimaging；fluorescent probes；hypochlorous acid；real water samples；rheumatoid arthriti

1引言

类风湿性关节炎（RA）是一种广泛存在的慢性全身性炎症性自身免疫性疾病，以软骨破坏和慢性关节滑膜炎为主要特征。（剩余15598字）