褪黑素抗慢性束缚应激致小鼠脑损伤研究

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中图分类号：S852.3 文献标识码：A文 章 编 号：1673 9868（2025）06 04 13

Abstract：Pscholoicalstresisoneofthemaorhealththreatsfacedb com anionanimalssuchasdos andcatsin moderndomesticenvironments.Lon-termexosuretohih-stres conditionscanleadto behavioralabnormalities，supresionofimmunefunction，andneurodegenerativediseases，seriously afectinganimalwelfareandqualityoflife.ThisstudyestablishedapsychologicalmodelinKunmingmice through28daysofchronicrestraintstres andthenintervenedwithexogenousmelatonin.Behavioral， ELISA， biochemical， histological and immunohistochemical techniques were used to evaluate the potential protectiveefectofmelatonin（MT）onbraininjurycausedbychronicrestraintstres （CRS）inmice.The results showed that CRS led to anxiety and depresion-like behaviors in mice， significantly increased the levels of CORT， NE， IL- ⋅1β ， IL-6 and TNF- ∇⋅α in the plasma and hipocampus of mice ϕ<0.05） ）， significantl decreasedthelevelsof5-HT，MTandDA ⋅p<0. 05⟩ ），andsinificantlincreasedthelevelofMDA inthe lasmaofmiceanddecreasedthelevelsofCAT，SOD，GSH- ⋅Px andT-AOC ）.Further studies found that CRS significantly reduced the proportion of positive expresion areas of prefrontal cortex andhipocampusneuronsandNeuNproteininmice ）.Incontrast，aftertheinterventionwith MT， the stres response and anxiety and depresion-like behaviors caused by CRS in mice were aleviated， thelevelofoxidativestresinthebod wasinhibited，andtheneuronsinthebrainofmicewas rotected， whichwasmanifestedasasinificantl decreaseinthelevelsofCORT，NE，IL- ，IL-6andTNF- ∇⋅α∇ inthe plasmaofmice ⋅p<0.05） ），anincreaseinthelevelsof5-HT，MT，DAandtheantioxidantenzymes including CAT， SOD and GSH- ⋅Px ${ ＼it p } < 0 . 0 5 ＼$ ）， thereby inhibiting the production of MDA and the reductionofthepositiveexpresionofNeuNprotein，andaleviatingthedegre ofpsychologicalstres of mice.Inconclusion，thisstud showsthatMThasa otentialtheraeuticefectonthebraininur caused byCRSinmice.

Keywords：mice;psychologicalstres;chronicrestraintstres;melatonin;prefrontalcortex;hipocampus

心理应激是伴侣动物（如犬、猫）在现代驯养环境中面临的主要健康威胁之一[1]。（剩余15603字）