玉米苗期抗旱IncRNA基因的挖掘

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Identification of Drought Resistant Associated IncRNA Genes in Maize Seedlings

FENG Xinyu ，LIU Xuyang，TANG Huaijun，HE Guanhua，LI Yongxiang，LI Chunhui， LIU Cheng，WANG Tianyu，LI Yu，LU Yuncai'，ZHANG Dengfeng

（College ofAdvanced Agricultureand Ecological Environment，Heilongjiang University，Harbin150080; ²Institute of Crop Sciences， Chinese Academy of Agricultural Sciences/State Key Laboratory of Crop Gene Resources and Breeding，Beijing l0oo81;3Institute ofGrain Crops， XinjiangAcademy ofAgricultural Sciences，Urumqi 830091）

Abstract：Drought is one of the major abiotic stresses afecting maize production in China.Long non-coding RNAs （IncRNAs）playcrucialrolesiplantresponses tovariousabioticstreses，includingdrought.Thisstudyrevealedthattheinbred line H082183exhibitedsignificantlyhigherrelative watercontent thanLv28under moderate drought streses atthesedingstage， indicatingsuperiordroughtresistanceinH082183.RNA-seqanalysisidentified56and91drought-responsivediferentialy expresed IncRNAs distinguishingLv28 and H082183 under moderateand severe drought stress，respectively.Gene Ontology （GO）enrichmentanalysis highlighted drought resistant associated terms，including stressresponse，dehydrationadaptatio，and ABAsignaling pathways.Key lncRNAsand lncRNA-target genes involved in drought resistancewere identified：TCONS

00065324，targetingASR3，potentialymediatingABA-dependentsignal transduction；TCONS_0o045452andTCONS00081959， targeting Zm0o0ld02336and Zm0old043368，encodingaWRKYandaTCPtranscription factor，respectively；TCONS 00023643，TCONS09553，TCONS00211191andTCONS00160812，targetinggenes encodingfunctioalproteinsaoiated with molecularchaperones，wax biosynthesis enzymes，andLEA（Late Embryogenesis Abundant）proteins.These lncRNAsand their corresponding target genes willserve as promising candidate genes for drought resistance in further study.

Keywords：maize;drought;lncRNA;transcriptomeanalysis

玉米（ZeamaysL.）是我国乃至全球最重要的粮饲兼用作物之一，干旱是影响玉米产量提高的最主要限制因素之一，常常导致产量严重下降甚至绝收[1-2]。（剩余20502字）