短绒野大豆居群地理空间遗传结构-居群间种质搜集策略研究

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Study on the Genetic Structure in Geographical Space of Glycine tomentella Hayata Populations and the Strategy for Germplasm Collection among Populations

WANGHaochen1'²，WANGKejing²，HANJuan1，LI Xianghua² （CollegeofAgriculture，NorthestAgriculture&ForestryUnivesityangling0hani;stituteofroience， ChineseAcademyofAgricultural Sciences，Beijing 100081）

Abstract：The perennial Glycine tomentella Hayata is only distributed in the southeast coastal zone of China.It was listed as anational key protected wild plantby the state in 2O21.How to reasonably and effectively conduct sampling among geographical populations is of great significance for the evaluation of genetic diversity and geographical variationof this species and thecolectionof germplasm resources.In this study，24 pairs of SSR markers with stable bands were selected from 400 pairs of species specific SSR primers，and 33 populations of G tomentell were analyzed by SSR.The results showed that the number of observed alleles ranged from 6 to 19，with an average of 11.83； The expected heterozygosity ranged from O.470 to 0.928；The multiple site outcrossing rate was low （ 0.3% on average）；The genetic variation among populations （22.33%） ）was less than that within populations （77.13% ），the average genetic differentiation coeficient among populations was low（ Gst =0.225 ），and hada high level of gene flow（ ）. Spatial autocorrelation analysis showed that there was a significant genetic correlation in the range of 125km . STRUCTURE analysis showed that 33 populations had two main genetic component background groups and were divided into three clusters using Nei's genetic distance NJclustering，which was basically consistent with the geographical distribution of northern，middle and southern populations. Fδ -statistics showed that each population had its own specific genetic structure. Therefore， according to the characteristics that the species is only distributed in the narrow coastal zone of Fujian and Guangdong in China，the high genetic variation within the populationand the significant genetic diferences among populations，， the proposed sampling strategy is to collect as many populations as possible.

Key words： Glycine tomentella ; genetic diversity ; genetic structure; SSR;sampling strategy

不同植物地理居群在长期的生长和演化过程中对当地的环境条件具有适应性，形成了独特的遗传特征。（剩余16658字）