青稞种质资源萌发期耐盐碱性的鉴定评价

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Evaluating the Variation of the Saline-Alkaline Resistance in Hulless Barley Germplasm Resources at Germination Stage

HAO Yilei， YAO Youhua，YAO Xiaohua，MA Hong，WU Kunlun，CUI Yongmei

（LaboratoryforResearchand UtilizationofQinghai Tibet Plateau GermplasmResources，AcademyofAgriculturalandForesty

Sciences，QinghaiUniversity/QinghaiKeyLaboratoryofHulessBarleyGeneticsandBreeding/QinghaiHullssBarleySubcenter ofNational TriticeaeImprovement Center，Xining810016）

Abstract：Saline-alkaline stress，a major abiotic constraint limiting the huless barley growth and development，underscores the importance of screning tolerant germplasm resources for comprehensive utilization of saline-alkaline land. In this study，333 huless barley germplasm resources were evaluated under saline-alkaline stress at the germination stage.Germination potential（GP），germination rate（GR），root length （RL），germ length （GL），root dry weight （RDW），and germ dry weight （GDW） were assessed to identify the key tolerance indicators and resilient varieties.Index variation analysis showed that GR was the most significantly inhibited parameter under saline-alkaline stress.Further validation using PLS-DA discriminant， Random Forest，and LASSO regression confirmed GR and GL as robust indicators highly correlated with salinealkaline stress.Through integrated D-value correlation and gray correlation analysis，GL exhibited the strongest association with stress response.Cluster analysis clasified the 33 germplasms into five distinct tolerance categories.Based on comprehensive D-value evaluation of GR and GL，thirteen extremelysaline-alkalinetolerant and ten saline-alkaline-hypersensitive varieties were identified.These extreme germplasms can serve as optimal parental lines for breeding programs，as well as provide foundational material for uncovering key regulatory genes and elucidating molecular mechanism governing saline-alkaline tolerance during critical growth stages of hulless barley.

Key words： hulless barley;saline-alkaline stress;germination stage;screening of germplasm resources

青稞（HordeumvulgareL.var. nudum Hook.f.），属禾本科小麦族大麦属，作为大麦的变种，是一类综合抗逆性较强的一年生作物。（剩余23009字）