NADPH氧化酶1在射血分数保留性心力衰竭中的表达特点及作用机制分析

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Expression Characteristics and Mechanistic Analysis of NADPH Oxidase 1 in Heart Failure with Preserved Ejection Fraction

LI Jinshuang1，WANG Miaomiao²，XU Ziheng1，GAO Xingjuan1，SHI Ce³ （1.DepartmentofCardiologyheAfiatedSuqianHospitalof Xuzhou MedicalUniversity，uqian2238O，Jiangsu，hina; 2.DepartmentofNursing，TheAfliatedSuqian HospitalofXuzhou Medical University，Suqian2238O，Jiangsu，hina; 3.DepartmentofOrtoedics，，he AfliatedSuqian Hospitalf Xuzhou Medical Uniersityuqian2238o，iangsuCina）

【Abstract】ObjectiveToexplorethe molecular mechanisms of Alarin/NOX1pathway intheprocessesofheartfailure with preserved ejectionfraction（HFpEF）MethodsDalsaltsensitiveratswereusedtocreateanimalmodelsofHpEF.Theratswererandomlydivided intoacontrolgroup，ahighsaltdietgroup，ahighsaltdiet+Alaringroup，ahighsaltdet+overexpresiocontrolgroupaghsaltdie + NOX1 overexpression group，and a high salt die + Alarin + NOX1 overexpression group，with 5 rats in each group. Changes in E/e’and left atrial area ofratsweredetectedbychocardiography.Thelevelsofreactiveoxygenspecies（ROS）and8-hydroxy-2'-deoxyguanosine（8-OHdG）in myocardialtisuewasdetectedbyELISAmethod.HEstainingwasusedtodetectmorphologicalchangesinmyocardialtissue. ImmunohistoccaldetetofarinndNX1protexpresioninmyoadaltss.Masosgsusdtetettheposio of collgefibs inyocadialisse.Teexpressoofryaodinereptor2（Ry）andsarcoplasmicetimcalimpup（SEC2） inmyocardialisseasdetecedbWesteblotResultsThlftatrialareaandE/e’indexsignificantlecreadinteighaltdiet Alarin group and the high sat diet+Alarin + NOX1 overexpression group.The high salt diet+Alarin group and the high salt diet+Alarin + NOX1 overexpressongroupsignificantlyreducedthecontentofROSand8-OHdGinthemyocardialtissueofrats.Thehighsaltdiet + NOX1 overexpressiodsdoalllsedolodallldd muscle fibers.Mason staining results showed that collgen fibers were most significantly increased inthe high salt diet + NOX1 overexpression group.Thehighsaltdiet+laringoupsignificantlyducedteexpresionevelofy2proteinintemyocardialtisueofatsilethe expresionlevelofSERCA2proteinsignificantlyincreased.ConclusionNOX1wassignificantlyincreasedinHFpEFats.NOX1combind with Alarin to improve oxidative stress，thus improving the cardiac function of HFpEF. 【Keywords】 Heart failure with preserved ejection fraction;NADPH oxidase1;Alarin; Oxidative stress

射血分数保留性心力衰竭（heartfailurewithpreservedejectionfraction，HFpEF）是指射血分数正常或接近正常（ ⩾50% ），但有症状或体征的心力衰竭（heartfailure，HF）[1]。（剩余10395字）