基于16sRNA测序技术研究重度三尖瓣关闭不全患者肠道菌群的变化

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The Changes of Intestinal Flora in Patients with Severe Tricuspid Regurgitation were Studied Based on 16sRNA Sequencing Technology

FANG Dou1，ZHANG Chaolong²， XIAO Shuo³，LI Yuxin1， WANG Zhenzhong1，²，ZHONG Lishan 1，2，3 ，HUANG Huanlei 1，2，3 （1.Department of Cardiac Surgery，Guangdong Provincial People's Hospital（Guangdong Academy of Medical Sciences）， Southern Medical University，Guangzhou51oo8o，Guangdong，China；2.Guangdong Cardiovascular Institute，Guangdong Provincial People’s Hospital，Guangdong Academy of Medical Sciences，Guangzhou 51008o，Guangdong，China; 3.School of Medicine South China University of Technology，Guangzhou 51ooo6，Guangdong，China）

【Abstract】ObjectieToaalyztehangesofintestialflorainpatientswitholatedsveretricuspidregurgitationndexplote feasibilityofmicrobiotainterventionthrapyintesepatients.MethodsPatientsithisolatedseveretricuspidregurgitationwowere continuouslyadmitedtoteDepartmentofCardiacSurgeryofGuangdongProvincialPeole’sHspitalfromSeptember2O toOctober03 were enrolledin the tricuspid regurgitation experimental group（group T， n=20 ）.Foreach patient in the group T，a patient with isolated severemitralregurgitationwhowashospitaliedforsurgeryasrandomlyselectedduringtesameperiodasthegroupformitralregiatio （group M，n=20 ）.Inaddition，foreverytwopatientsenrolld intheexperimentalgroup，ahealthyadult wasselectedasthehealthycontrol group （group N，n=10 ）.Stool samples from three groups were colected and then subjected to 16sRNA gene sequencing. Bioinformatics analyses were performed according to the sequencing results.ResultsAnalysis of α diversity showed that the index of Simpson，Sobs，Chao1， ACEof intestinalflorainTgrouplower thanthoseinMgroupandNgroup，butthere wasnostatisticallsignificantdiference P>0.05）. The β diversityshowedthatthethreegroupswereseparatedfromeachother.Corelationanalysisshowedthattherelativeabundanceof Ruminococcaceae，Oscillospirales，Ruminococcus_sp N15MGS-57，and Roseburia - inulinivorans were positively correlated with tricuspid anularplanestolicecusioCoclusioeitestialfoaisstubedinpatitsiteeretrcuspidgugiatiode significantcoeaenultileootasndigartfunctoindesiseatints，sugtigatitestifbe associated with the occurrence and development of severe tricuspid regurgitation.

【Keywords】 Severe tricuspid regurgitation;Intestinal flora;Heart failure;16sRNA sequencing

三尖瓣关闭不全是一种常见的心脏瓣膜疾病，因其病程的隐匿性使疾病未得到足够的重视。（剩余9984字）