ALKBH5介导的 m6A 修饰在早孕小鼠子宫内膜蜕膜化中的作用研究

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【中图分类号】R114【文献标志码】A

Roleof ALKBH5-mediated m6A modification in endometrial decidualization of mice in early pregnancy

HeSihui，Li Weike，Gao Rufei，Chen Xuemei，Long Jing，Chen Chenxi，Dai Dexian，Wang Yingxiong，Li Na （Joint International Research Laboratory of Reproduction and Development，Ministry of Education， School of Public Health，Chongqing Medical University）

【AbstractObjective：Toinvestigatetheroleoflkylationrepairhomolog5（ALKBH5）-mediatedN6-methyladenosine（m6A）modification inendometrialdecidualizationMethods：Themouse modelsofpregnancyandpseudopregnancyereestablished，ndquantitativereal-timePCRandWestenblotwereusedtomeasuretheexpresiopattofALKB5inthendometrum.Themouseadcell modelsofartificiallduceddidualzationeretablsd，nduantitatiereal-teCR，Wstenblot，ndimmunistotrywereused to measuretheexpressionlevelsofdecidualization-relatedmarkers.TheEpiQuikm6ARAmethylationquantification kitwasused tomeasurethelevelofm6A.Themouseandcellmodelsofartificiallyinduceddecidualizationwithinterferenceof ALKBH5 expressnwereablisd，ndquantitatieal-ePCR，Westenblot，ndmmuoistohemistryresdtoasure thexpressonlevelsofdecdualizationrelatedmarkers，cellprolferationmarkermoleculesndapoptosisolecules.Flowcytomety wasusedtomeasurecellapoptosisate.Results：InthemousemodelofpregnancytheexpresiolevelofALKBH5attheuterineebryoimplantationsiteassignificantlyhighertanthatadjacenttoteiplantationite，andintemouseandcellmodelsofartificiallinduceddecidualizationcompaedwiththeontrolgroup，thinductiongrouphdasgnificantincreaseintheexpressionlevel of ALKBH5andasignificantreductioninthelevelofm6A.InhibitingtheexpresionofALKBH5ledtoanincreaseinthelevelof m6A，whicintuitedeprolferatioofroalcelsduedellpoptosisdulatelyimpiedtoalprof dometrialdecidualization.Conclusion：ALKBH5deficiencyleadsto anincreasein thelevel of m6Aand decidualizationinjuryinthe endometrium，whichlaysafoundationfortheresearchonm6Amodificationindecidualization.

【Key words】alkylation repair homolog 5;N6-methyladenosine;endometrial decidualization;cell proliferation;cell apoptosis

人口负增长已形成难以逆转的趋势，女性妊娠成功率降低是人口下降的重要原因。（剩余13947字）