cGAS-STING信号通路在不同程度冠状动脉粥样硬化病变下的表达研究

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【中图分类号】R89 【文献标志码】A

【AbstractObectiveToobsevethepathologcalchangesofmyocardialtisseunderdiferentdegresofcoronaryatheroseoticlesions，tomeasue theexpressionlevelsofproteinsaoiatedwiththcyclicGMP-AMPstase（cGAS）stimulatorofinteferones （STING）signalingpathwayincoronaryarteries，andtoinvestigatetheroleofcGAS-STIGinthedevelopmentand progresionof coronaryheartdisease.Methods：Eligiblecasesofcoronary heartdiseaseandcontrolcases wereselectedanddivided intocontrol group with normal coronary arteries and grade I，I，I，and IV coronary artery stenosis groups.HE staining was used to observe and evaluate tepathologicalconditionsofcoroaryarteries，Westeblting wasusedtomeasure theexpressionlevelsofdownsrampro teinsoftheGASSTINGpathwayincoronarytisue，ndELISAwasusedtomeasue thelevelsofinflammatorfctorsinoroayissue.AcorrelationanalsisaspeformedtoinvestigatethecorelationoftheexpressonlevelsofdownstreamproteinsoftheGASSTING signalingpathwayandrelatedinflammatoryfactors withthedevelopmentandprogresionofcoronaryheartdisease.Results：Microscopicexaminationshowedthatcomparedwiththecontrolgroup，theotherfourgroupshadvaryingdegesofpathologicalchanges suchas vascular wall thickening and luminal stenosis，with a gradual increaseinthedegree of stenosis from grade I to grade IV coronarylesions.Comparedwith thecontrol group，the grade l，ll， （204 III ，and IV coronary artery stenosis groups had significant increases in the expression levels of downstreamproteins of the cGAS-STING signalingpathwayand related inflammatory factorsincoronary tissue，with a trend of increase from grade I to grade IV coronary lesions.TheexpresionlevelsofdownstreamproteinsoftheGASSTIGsignalingpathwayincoronarytissuewerepositivelyceated with the development and progression of coronary heart disease（cGAS： r=0.927 P<0.001 ： -Sting： r=0.889 ， P<0.001 p -TBK1： r= 0.910，P<0.001 -IRF3： r=0.936 P<0.001 ;IFN-1： r=0.936 P<0.001 ;TNF- α⋅α∝ r=0.945 ， P<0.001 ;IL-1β： r =0.962，P<0.001;IL-6：r= 0.933， P<0.001 ）.Conclusion：There are significant differences in the expression levels of downstream proteins of the cGAS-STING signalingpathwayandrelatedinflammatoryfactorsndthissignalingpathwayaybeapotentialargetforthreatmentofooary heart disease.

【KeyWords】coronary artery;inflammatory factors;cyclic GMP-AMP synthase

冠心病（cornaryheartdisease，CHD）是一种常见且严重的心血管疾病。（剩余11846字）