hnRNPA2B1介导 m6A 修饰MIR100HG促进胃癌MKN-28细胞增殖和侵袭作用
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【中图分类号】R56
【文献标志码】A
【Abstract】Objective ：To investigate the expression of N6 -methyladenosine（ m6A ）reader heterogeneous nuclear ribonucleoprotein A2/
B1（hnRNPAB1）inhumangastriccancer（GC）tissueanditseffectontheproliferationandinvasionofMKN-28cells.Methods：he
Cancer Genome AtlasandGene Expresson Omnibus wereusedtoanalyze theexpressonofhnRNPA2B1andlong noncoding RNA（ln
cRNA）mir-100-let-7a-2-mir-125b-1clusterhostgene（MIR100HG）inGCtissueandtheirassociationwiththeclinicalprognosis
of patients with GC.QuantitativePCRand Westernblotingwereused to measuretheefectofhnRNPA2Blontheexpressonlevelof
MIR10OHGanditsdownstreamWnt/β-cateninsignaling pathway；MethylatedRNAimmunoprecipitation（MeRIP）wasusedtomea
sure the m6A levelofMIR1OoHG;CCK-8 assayand Transwellassay were usedtoobserve cellproliferationand invasion.Results ：Com
paredwith paracancerous tissue，human GC tissueshowed significant increases in theexpression levelsof hnRNPA2B1（ t=6.101，P< （204号
0.001）and MIR100HG（ t=2.191 P=0.036 7 ），and the high expression levels of hnRNPA2B1 and MIR1OoHG were associated with
poorsurvivalinpatients withGC.KnockdownofhnRNPA2B1reducedthemRNAexpresionlevel（t=5.156，P=0.007）andmAlevel
of MIR100HG（t=4.789， P =0.010），inhibited the proliferation and invasion of MKN-28 cells（ t=4.915 ，P=0.008 and t=5.167，P= 0.007），and blocked the activity of the Wnt/β -catenin signaling pathway（ P <0.05）. Overexpression of MIR100HG promoted cell proliferation andinvasion（ t= 3.578， P =0.023and τ =8.411， P =0.001）， activated theWnt/β-catenin signaling pathway（ P <0.01），and reversedthe antitumor effectinducedby hnRNPA2B1knockdown（ t=3.667 P=0.021 ）.Conclusion：This study shows that hnRNPA2B1- mediatedm6Amodificationof MIR10oHG promotes the proliferationand invasionofGCMKN-28cellsbyactivatingtheWnt/B -catenin signaling pathway.
【Key words】heterogeneous nuclear ribonucleoprotein A2/B1;gastric cancer;proliferation
胃癌是最常见的胃肠道恶性肿瘤之一，在全球范围内发病率排第5，癌症相关死亡率排第 3[1] 。（剩余9300字）
