大柴胡汤缓解葡聚糖硫酸钠诱导的溃疡性结肠炎及肝损伤并与其对小鼠肠道菌群的调控作用相关

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【中图分类号】R285.5 【文献标志码】A

【AbstractObjective：To investigate the preventiveand therapeutic efectsand mechanismsofDachaihudecoction（DCD）ondextran sodiumsulfate（D）-ducedulcerativecolitis（UC）andiverijuyince，aswellasthesociatonbtwenCDefitsadgt microbiota modulation.Methods：Mice weretreatedwith DCD（20.10and10.05g/kg）for2 weeks，withfree accesstodrinking water containing 3% DSS in the second week to induce UC. Histopathological examination，RT-qPCR and 16S rRNA sequencing were used toinvestigatethefectofDCDonUCmice.Results：DCDpretreatmentsignificantlyaleviatedweightloss，，bloodydarwith mucus，istopathologicalboalitisofteolondcolonsorteinginiceitSinduedUC.Idditioneat mentsignificantlyupregulatedthlevelsofOccudin，ZO-1，andMUC-2inthecolonandprotectedtheintestialbarierofic.DCD pretreatmentalsolviatediflammatorycellifilrationinolodtelivdsigiicantldudteexpreiolelsof

the proinflammatory factors such as IL- 1β ，IL-6，TNF- α ，iNOS， COX-2，and NLRP3，thereby exerting a protective effect against UC andliver injury. It should be noted that DCD corrected gut microbiotaimbalanceinUCmicebyenrichingprobioticbacteriasuchas Lactobacillusand Bifidobacteriumand reducingharmful bacteria suchas Norank_f_Desulfovibrionaceae and Escherichia-Shigella. Conclusion：DCD canalleviateDSS-induced UC and exertaliverprotecting effectbyprotecting intestinal barrier，inhibitinginflammation，and regulating gut microbiota. 【Key words】Dachaihu decoction;ulcerative colitis ;intestinal barrier;gut microbiota;liver injury

溃疡性结肠炎（ulcerativecolitis，UC）是一种以黏膜上皮细胞损伤和肠道稳态失衡为特征的慢性复发性肠道炎症性疾病（inflammatory bowel disease，IBD），累及结肠和直肠[1-2]，被认为是一种现代难治性疾病。（剩余21654字）