TRPV1介导小胶质细胞自噬在带状疱疹后神经疼痛中的作用和分子机制

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【中图分类号】R741.02 【文献标志码】A

【AbstractObectiveToinvestgatethmechansmoftransientreceptorpotentialvaniloidsufamily（TRPV）-mediatedmicroglia autophagyinpostherpeticneuralgia.Methods：Fortymicewererandomlydividedintocontrol group，postherpeticneuralgia（PHN） group，PHN-sh-NC group，and PHN-sh-TRPV1group，with10 miceineachgroup.Wetested the mice'smechanical withdrawal threshold（MWT）and thermal withdrawal latency（TWL）;measured serum levelsof tumor necrosis factor-alpha（TNF- α ），interleukin （IL）-1β，IL-6，andbrainderivedeurotroicfactorBD）byee-lnkedimmunosorbentassa；assessdtheforatioofautophagosomesinthespialcord issuesbytransmissonelectronmicroscopy；measuredtheexpressonofmicrotubuleassociaedpro teinllightchain3（LC3）andionizedcalciumbindingadaptormolecule1（Iba-1）inthespinalcordtissesbyimmunofluorescence assay；and determined the protein expression of Beclin-1， microtubule-associatedprotein1lightchain 3B（LC3B），and p62 inthe spinal cordtissuesbyWesternblot.Results：Comparedwith the control group，the PHN group and PHN-sh-NC group had significantdecreasesinMWT，serumBNDF1 evel（t=10.49，P<1 0.001）， and p62（ P =0.004）protein expression in the spinal cord tissues and significantincreasesin TWL，serum TNF- α （t=26.27， P <0.001）， IL-1β（t=17.0，P<0.001），andIL-6levels（ ⋅ =25.48，P<0.001），and the expression of Iba-1（ P=0.002 ），LC3（ P<0.001 ），LC3B（ P=0.001. ），and Beclin-1 proteins（ P=0.001 ）in the spinal cord tissues. ComparedwiththePHNgroup，thePHN-sh-TRPV1grouphadasignificantlyhigherMWT，asignificantlyhigherserumBNDFlevel （t=5.174， P<0.001 ，a significantly higher p62 protein expression level （ P<0.001 ）in the spinal cord tissues，a significantly lower TWL， significantly lower serum TNF- α （ t=20.57 P<0.001 ），IL-1β（ t=8.260 P<0.001 ），and IL-6 levels（ t=19.81 ， P<0.001 ），and significantly lower expression levels of Iba-1（ P<0.001 ），LC3（ P<0.001 ），LC3B（ P=0.001 ），andBeclin-1（ P<0.001 ）in the spinal cord tissues.Conclusion：MirogliautophagyisactivatedinthespnalcordofPHNmice，andsuppressingtheexpressonofTVcannhibit microglia autophagy to relieve pain in PHN mice.

【Key words】TRPV1;microglia autophagy ;postherpetic neuralgia

带状疱疹后神经痛（postherpeticneuralgia，PHN）是一种顽固性神经病理性疼痛疾病，主要症状为自发痛、诱发痛、痛觉过敏等。（剩余11862字）