干旱胁迫下芋根系转录组分析

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中图分类号：S632.3 文献标识码：A 文章编号：1000-4440（2025）11-2097-1（

Abstract：To explore the molecular response of taro roots to drought stress，this study used Lipu Taro No.1 as the material，sampledtarootsuderdroughtstress，andperformedtranscriptomesequencing.Bycomparingthedierences in gene expresion between the drought group andthecontrol group，as wellas conducting GO enrichment analysis and KEGG enrichmentanalysis，thekeypathwaysof taro roots inresponsetodroughtstresswere identified.Furthermore，genesrelated to these keypathways were identified，and their expresion paterns underdrought stress wereanalyzed.Theresults showed thatatotal of1O63diferentiallyexpressed genes （DEGs）were identified intaro roots underdrought stress，including 473 up-regulated genesand59Odown-regulatedgenes.TheGO enrichmentanalysisindicatedthat the prominentbiologicalprocesses enriched by DEGs were cellular processes and metabolic processes.The most enriched cellular component wascellularanatomical entities，and theprominent molecularfunctions were catalytic activityand binding processes.The KEGG enrichment analysis showed that themost significantly enriched pathway for the differentiallyexpressed genes was the biosynthesis of secondary metabolites.Through statistical analysis of the number ofDEGs inthemitogen-activated protein kinase（ MAPK ）signalingpathway and carotenoid biosynthesispathway，15 and four up-regulatedDEGs，and12andonedown-regulated DEGswere identified，respectively.Theanalysisrevealed thattheexpression of genes related to the MAPK signaling pathway and carotenoid biosynthesis pathway was affected by drought stress. MAPK （204号 may respond to drought stressby interacting with abscisicacid （ABA）and reactive oxygen species（ROS）signaling pathways，whilecarotenoids mayrespond todrought stressbydecomposing to produce organicsubstances andserving as a precursorofABA.Theresultsofthisstudylayatheoreticalfoundationforresearchonthemolecular mechanismsofdroughtresponse in taro and provide a valuable resource for breeding drought-resistant cultivars.

Key words：taro；drought stress；transcriptome sequencing；MAPK signaling pathway；carotenoid biosynthesis pathway

芋[Colocasiaesculenta（L.）Schott]，也称芋头，天南星科芋属多年生草本植物，原产于热带沼泽地，喜温暖湿润气候[1]。（剩余14109字）