中药复方发酵液抗猪沙门氏菌的药理机制

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中图分类号：Q815；R96 文献标志码：A

Abstract：Tovestigatethepotentialandthemechanisms ofactionoftheChineseherbalformula intreating porcine salmonelosis，，network pharmacologywasadoptedtoscren theactivecomponentsof thecompound，moleculardockingtechnology wascombined tovalidate target bindingactivity，and in vitroantibacterial experiments wereconducted to evaluate the antibacterial efect.Based on Traditional Chinese Medicine SystemsPharmacologyDatabaseandAnalysis Platform （TCMSP），active compound ingredients of the formula were acquired，GeneCards and Online Mendelian Inheritance in Man（OMIM）databases were integrated to screen disease-related targets.STRING database was utilized to construct protein-protein interaction networks，and Cytoscape3.1O.1software was employed fortopologicalanalysis to identifyprimary targets.DAVID database was adopted tocomplete gene ontology（GO）functional anotation and Kyoto Encyclopedia of Genes andGenomes （KEGG）pathway enrichment analysis.The results show that the Chinese herbal formula contains 2 273 active components and 2O6 intersecting targets，among which glyceraldehyde ⋅-3⋅ -phosphate dehydrogenase （GAPDH） is the prmartarget.Theactive ingredientkaempferolbinds stably to GAPDH，andtheChinese herbalformula fermentation significantlyinhibitstheproliferationofSalmonellacholeraesuis.Theantibacterial mechanismofaction involves the regulationof the phosphatidylinositol3-kinase/protein kinaseB（PI3K/Akt）signaling pathway，revealingthatthe Chinese herbal formulaexertsitsantibacterialefectthroughamulti-componentsynergisticregulationofmulti-target-multi-pathway interactions.

Keywords：network pharmacology；kaempferol；molecular docking；in vitroantibacterial experiment；pharmacological mechanism

猪沙门氏菌病作为猪群中一种高发性且高传染性的细菌性疾病，其病原体为沙门氏菌属Salmonellaspp.中的特定血清型。（剩余12218字）