独脚金内酯对葡萄夏芽萌发和相关基因表达的影响

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中图分类号:S663.1 文献标识码:A
Effects of Strigolactones on The Germination of Grape Summer Buds and The Expression of Related Genes
GE Yaqi,LIU Meng,DU Jingrong,WANG Yang,ZHANG Chaohong (l. College of Horticulture,Northwest A&F University,Yangling Shaanxi 7121Oo,China; 2.Southwest Forestry University,Kunming Yunnan 65O224,China)
Abstract:【Objective】Theefects of strigolactone on the germinationof grape summer budsand the expressionof strigolactone synthesis pathwayandsignal ransduction genes inbuds werestudied,which providedatheoreticalbasisfortherational useof strigolactone anditsinhibitionofsummer bud germinationin grape production.【Methods】Taking‘Liaofeng’grape as test material,the germination number,lengthand stem diameterof summer buds were investigated byapplying diferentconcentrations of strigolactone analogue GR24,andtheexpressionof synthetic genes inbuds was determined.【Results】Exoge nous GR24 hadasignificant inhibitory effecton the germinationof grapesummerbuds.With the increaseof hormoneconcentration,theinhibition time wasprolonged.However,the efect on the growthof secondary shoots after germination was not obvious.After GR24 treatment,the expresion levels of VvD27,VvMAX1,VvCDD7andVvCDD8decreased withthe increaseof concentrationThe expresion levels of monostearin signal transduction genes VvMAX2andVvDl4 increased with theincreaseof treatmentconcentration.【Conclusion】Theresultsshowed that strigolactonecouldefectivelyinhibitthegerination of summerbuds.Itmayafectthegermination andgrowthof summerbudsbyaecting the synthesisand signal transduction of strigolactone in grape summer buds.
Key words: grape;strigolactone;summer bud; germination rate; gene expression
葡萄的栽培历史悠久,是世界范围的重要果树[]。(剩余6955字)