微小RNA-302a-3p靶向溶酶体相关膜蛋白5抑制口腔鳞状细胞癌侵袭转移的研究

打开文本图片集

[中图分类号] R739.8 [文献标志码] A [doi] 10.7518/hxkq.2025.2024409

[Abstract]ObjectiveThis study aimed to explore the expresionof lysosomal-assciated membrane protein 5 （LAMP5）and microRNA （miR）-302a-3p inoral squamous cellcarcinoma（OSCC）and their functional mechanism on the invasionand metastasis ofOSCC.MethodsThe expressionofLAMP5 in OSCCandits sensitivity asa prognostic indicator were analyzedon thebasis ofThe Cancer GenomeAtlas database.Western blot，quantitativereverse transcriptionpolymerasechainreaction，andcellimmunocytochemistry wereused todetect theexpressionofLAMP5 inOSCC tisuesandcell.TheeffctofLAMP5ontheproliferation，migration，andinvasionofOSCCcellswasevaluated throughcellcountingkit-8，immunocytochemistrymigration，andinvasionassays，respectively.ThemiNAtargeting predictionwebsiteswereusedtopredictthe miRthatregulates LAMP5andverifythe targetedregulatoryefectof miR302a-3pon LAMP5.The effectofLAMP5 knockdownonOSCC tumor growth was evaluated inanude mouse tumorigenesis model.ResultsLAMP5was highlyexpressed inOSCC tisuesandcels.It showed high sensitivity intheearly diagnosisofOSCC.LAMP5knockdownsignificantly inhibitedtheproliferation，migration，and invasionofOSCCcells， whereasLAMP5overexpression increased thesecellactivities.TheexpressionofLAMP5wasregulated by miR-302a3p.In vivo，LAMP5knockdownsignificantly inhibited thegrowthofOSCCtumor.ConclusionLAMP5promotes the malignant progressionofOSCCbyenhancing theproliferation，migration，and invasionofOSCCcels.Theexpression ofLAMP5 is negatively regulated by miR-302a-3p.

[Keywords] microRNA-302a ⋅3p ；lysosomal-associated membrane protein 5； oral squamous cell carcinoma；invasion and metastasis

口腔鳞状细胞癌（oral squamouscellcarcino-ma，OSCC）是最常见的头颈部恶性肿瘤之一，约占口腔癌的 90% 以上[-2]。（剩余15524字）