湖南金丝皇菊花叶病害病原物鉴定

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中图分类号：S682；S432 文献标识码：A 文章编号：1006-060X（2025）06-0090-06

引用格式：，等.湖南金丝皇菊花叶病害病原物鉴定[J].湖南农业科学，2025（6）：90-95.DOI：10.16498/j.cnki.hnnykx.2025.006.016

Identification of Pathogens Causing Mosaic Disease of Chrysanthemum morifolium 'Jinsi Huangju' in Hunan Province

HOU Jia-yi 1，2，3 ，LI Ling'，CHEN Xue-feng1，WU Bo-shun1，XIANG Li-li1，2³，YU Xiao-ying 1，2，3 ， XU Lu12.：

（1.Colegeoficuue，aricalUstsh，;.ndropalQality andUtilizationeinoeahntegh8C;3esnbratorygsha）

Abstract：To identifythe pathogens causingtypical mosaicdiseaseofChrysanthemum morifolium'Jinsi Huangju’inHunan，we colected the leaves withmosaic diseasesymptoms from the Hongcichrysanthemum plantingbase.RT-PCRamplification，enzymelinkedimmunosorbentassay（ELISA），andnegativestaining electronmicroscopywereemployedtoidentifythepathogensithee dimensions ofucleotide sequence，morphology，and protein specificity.RT-PCRamplifcationofthediseased leaves producedtwo targetbands，which wereat thesizessimilartothetarget gene lengthsofchrysanthemum chlorotic motleviroid（CChMVdand chrysanthemum virus B（CVB）.ELISA confirmed the presenceof CVB-specific protein in the diseased leaves.Negative staining of the suspension preparedwiththediseasedleavesrevealedpathogencomponentintwodiferentshapes，whichweresimilarto morphologicalstructureofCChMVdandCVB.Thephylogenetic treeshowcasedthattheisolate（viroid）fromHunansharedthesame clade with four reference isolates （e.g.， CChMVd，FJ647546.1） from Spain， with the sequence similarityof 87% .In addition， the isolate （virus）fromHunansharedthe sameclade withthat fromNanjing （CVB，JQ904595.1），withthe sequencesimilarityof 100% .In conclusion，CChdandCBarethepathogenscausingmosaicdiseaseofC.morifoliumJinsiHuangju'inHunan，andteobied infection of the two viruses leads to the occurrence of this disease.

Key Words：Chrysanthemum morifolium'Jinsi Huangju'; virus identification; CChMVd; CVB; RT-PCR

金丝皇菊（Chrysanthemummorifolium'JinsiHuangju'）为药食兼用型大花茶菊，不仅富含黄酮类、多糖和氨基酸等多种药用活性成分，还极具观赏价值[1-3]。（剩余7384字）