烟草NtJAR1家族基因的鉴定及生物信息学分析

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引用格式：，杨梦璇等．烟草NtJARI家族基因的鉴定及生物信息学分析[J].湖南农业科学，2025（6）：8-15.DOI：10.16498/j.cnki.hnnykx.2025.006.002

中图分类号：S572；Q811.4 文献标识码：A 文章编号：1006-060X（2025）06-0008-08

Identification and Bioinformatics Analysis of NtJAR1 Family Genes in Nicotiana tabacum L.

ZHANG Zhao-ting¹，YANG Meng-xuan²，XU Fang-zheng²，MAO Dong-ping³，LI Shi-jin³， WANG Qian²，XU Gang³，NING Yang²

（1.Xuanzoutictccdstrvelote9;sechd of Agricultural Sciences， Qingdao 266lo1，PRC; 3.Anhui Wannan Tobacco Co.，Ltd.， Xuancheng ，PRC）

Abstract：This study aims to reveal thephytohormone metabolismand stressresponse mechanisms of flue-cured tobacco. Bioinformatictools wereusedtoidentifythejasmonoyl-isoleucinesynthetase （JARl）genesfromthewholegenomedataofNicotiana tabacumL.Thephysicochemical propertiesofthededucedproteins，phylogeneticrelationship，conserveddomains，genetructures， corecis-actingelemntsinthpromoteregiondtransiptioalpaes werealyed.Atotalof1NAgeeseetie inthegenomeofN.tbacumL.andtephylogenetictreeshowedthatesegenes wereclasifiedintotwosubfamilies （and）.The deducedproteishadthelengthsof360630aa，isoelectricpontsof5.566.93，andmolecularweightsof40.2-68.94KDa.They wereamphiphilicproteis withconsistentthemostabityExceptNtAR1-6，NAR1-3，NJAR1-9，andNAR1-12werelocatedin thenucleusotherproteisereintecoplasm.AlltheNJARlgneshadauiqueconseeddomainofteGH3fmily-5os， and2-5intros.TheNtJARlgenefamilymembersdemonstratedatissue-specificexpresionpateinN.tabacumL.Specificaly NtJAR1-5，NA-6，NA-8，NtJAR-10，NA-7ndNJAR-4wereighlyexpressedintheots，whileNA-d NJAR1-12wereghyepresedieave.NAgesespodedtoolds，ih-7-8，Nd NtJAR1-12 being up-regulated under cold stress.

Keywords：JAR1;tobacco （Nicotiana tabacumL.）;cold stress;bioinformaticanalysis;expressonpattern

茉莉酸（Jasmonicacid，JA）是一种广泛存在于植物体内的重要激素，主要参与植物生长和发育的调节[1-2]。（剩余10509字）